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首页> 外文期刊>Acta Pharmacologica Sinica >Acetazolamide suppresses tumor metastasis and related protein expression in mice bearing Lewis lung carcinoma
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Acetazolamide suppresses tumor metastasis and related protein expression in mice bearing Lewis lung carcinoma

机译：乙酰唑胺抑制Lewis肺癌小鼠的肿瘤转移及相关蛋白表达

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AIM: To study the suppressing effect of acetazolamide on tumor metastasis in vivo and observe the protein alteration of lung in mice bearing Lewis lung carcinoma. METHODS: The functional role of aquaporin-1 (AQP_1) was investigated in tumor tissues by SDS-PAGE and Western blot. The effect of acetazolamide on tumor metastasis was analyzed by Lewis-lung-carcinoma model. Differential protein was identified by SDS-PAGE, isoelectrofocusing (IEF) methods, and peptide mass fingerprinting (PMF). RESULTS: Acetazolamide (40 mg·kg~(-1)·d~(-1) po for 21 d) dramatically reduced the numbers of lung metastasis after sc inoculating Lewis lung carcinoma. The inhibition rate of lung metastases was 83.9 %. Simultaneously, the AQP_1 protein level and actin-cytoplasmic in lungs containing metastatic tumor deposits were found to be higher than that in the normal tissue. After treated with acetazolamide for 21 d, the expression of AQP_1 was obviously inhibited. CONCLUSION: Acetazolamide can suppress tumor metastasis, at least in part, by inhibiting the expression of AQP_1. AQP_1 and actin-cytoplasmic may be new prognostic molecules as well as new therapeutic targets for the prevention and treatment of metastatic tumor.
机译：目的：研究乙酰唑胺对体内肿瘤转移的抑制作用，并观察Lewis肺癌小鼠的肺蛋白变化。方法：通过SDS-PAGE和Western blot研究Aquaporin-1（AQP_1）在肿瘤组织中的功能。用路易斯-肺癌模型分析了乙酰唑胺对肿瘤转移的作用。通过SDS-PAGE，等电聚焦（IEF）方法和肽质量指纹图谱（PMF）鉴定差异蛋白。结果：乙酰唑胺（40 mg·kg〜（-1）·d〜（-1）口服21 d）可显着减少sc接种Lewis肺癌后的肺转移数量。肺转移的抑制率为83.9％。同时，发现含有转移性肿瘤沉积物的肺中的AQP_1蛋白水平和肌动蛋白胞质水平高于正常组织。乙酰唑胺处理21 d后，AQP_1的表达受到明显抑制。结论：乙酰唑胺可通过抑制AQP_1的表达至少部分抑制肿瘤转移。 AQP_1和肌动蛋白胞质可能是预防和治疗转移性肿瘤的新的预后分子和新的治疗靶标。

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