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首页> 外文期刊>Acta Pharmacologica Sinica >Caspase 3 gene expression and [Ca~(2+)]_i homeostasis underlying desipramine-induced C6 glioma cell apoptosis
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Caspase 3 gene expression and [Ca~(2+)]_i homeostasis underlying desipramine-induced C6 glioma cell apoptosis

机译：Caspase 3基因表达与地昔帕明诱导的C6胶质瘤细胞凋亡相关的[Ca〜（2 +）] _ i动态平衡

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AIM: To study desipramine (Des)-induced apoptosis and regulation of caspase 3 gene expression and [Ca~(2+)]_i homeostasis in rat glioma C6 cells. METHODS: Apoptotic DNA breaks were quantified by propidium iodide (PI) incorporation using flow cytometry (FCM) and were detected by DNA agarose gel electrophoresis. Expression of apoptotic effector gene caspase 3 was assessed by reverse transcription polymerase chain reaction (RT-PCR). Single cell [Ca~(2+)]_i was measured using fluorescence indicator Fura-3/AM with confocal laser scanning microscopy. RESULTS: Des induced apoptotic DNA breaks in a concentration-dependent manner evidenced by hypodiploid peak on FCM histogram and the apoptotic cell percentage induced by Des 10, 20, and 40μmol/L for 24 h was 5.2 %, 21.9 % , and 41.9 %, respectively. Apoptotic DNA breaks were further confirmed by a typical "DNA ladder" on agarose gel electrophoresis after exposure to Des 40μmol/L for 24 h. Meanwhile, expression of caspase 3 gene was observed following Des 20μmol/L treatment. Des 40μmol/L resulted in an early sustained increase in [Ca~(2+)]_i over 28 min and the elevation magnitude was greatly decreased by removal of extracellular free [Ca~(2+)]_i with calcium-chelator egtazic acid, suggesting that Des elicited [Ca~(2+)]_i influx rather than intracellular calcium mobilization. CONCLUSION: Up-regulation of caspase 3 gene expression and disturbance of homeostasis in calcium signaling system might play pivotal roles in Des-induced apoptotic DNA breaks of C6 cells.
机译：目的：研究地昔帕明（Despramine，Des）诱导的神经胶质瘤C6细胞凋亡及对caspase 3基因表达和[Ca〜（2 +）] _ i稳态的调节。方法：通过流式细胞术（FCM）通过碘化丙啶（PI）掺入定量凋亡的DNA断裂，并通过DNA琼脂糖凝胶电泳检测。通过逆转录聚合酶链反应（RT-PCR）评估凋亡效应基因胱天蛋白酶3的表达。使用荧光指示剂Fura-3 / AM和共聚焦激光扫描显微镜测量单细胞[Ca〜（2 +）] _ i。结果：Des诱导的凋亡DNA断裂呈浓度依赖性，这在FCM直方图上的二倍体峰证明，并且Des 10、20和40μmol/ L诱导24 h的凋亡细胞百分比分别为5.2％，21.9％和41.9％，分别。暴露于Des40μmol/ L 24 h后，在琼脂糖凝胶电泳上通过典型的“ DNA阶梯”进一步确认了凋亡的DNA断裂。同时，在Des20μmol/ L处理后观察到caspase 3基因的表达。 Des40μmol/ L导致[Ca〜（2 +）] _ i在28分钟内早期持续增加，并且通过用钙螯合剂依他唑酸去除细胞外游离[Ca〜（2 +）] _ i大大降低了海拔高度，表明Des引起[Ca〜（2 +）] _ i大量涌入，而不是引起细胞内钙动员。结论：Caspase 3基因表达上调和钙信号传导系统稳态紊乱可能在Des诱导的C6细胞凋亡DNA断裂中起关键作用。

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