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Inhibitory effect of sodium quercetin monosulfate on pig platelet aggregation induced by thrombin

机译:槲皮素单硫酸钠对凝血酶诱导的猪血小板聚集的抑制作用

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AIM: TO study the inhibitory effects of sodium quercetin monosulfate (SQMS) on pig platelet aggregation induced by thrombin. METHODS: Platelet aggregation was analyzed by turbidimetry. Cytosolic free calcium concentration ([Ca~2+]_i) was Determined by Fura-2 fluorescence. Activity of protein Kinase C (PKC) was assayed by incubating PKC with Histone Ⅲ S and [γ-~32P] ATP. The cytoskeletal Proteins were precipitated by Triton X-100 and Separated by SDS-PAGE. CONCLUSION: SQMS inhibited pig platelet aggregation induced by thrombin and its mechanism might be due to its Inhibitions of Ca~2+ influx, internal Ca~2+ release, PKC Activity, and actin polymerization.
机译:目的:研究槲皮素单硫酸钠(SQMS)对凝血酶诱导的猪血小板聚集的抑制作用。方法:采用比浊法分析血小板聚集情况。通过Fura-2荧光测定胞浆中游离钙的浓度([Ca〜2 +] _ i)。用HistoneⅢS和[γ-〜32P] ATP孵育PKC,测定蛋白激酶C(PKC)的活性。用Triton X-100沉淀细胞骨架蛋白,并通过SDS-PAGE分离。结论:SQMS抑制凝血酶诱导的猪血小板聚集,其机制可能是由于其抑制Ca〜2 +内流,内部Ca〜2 +释放,PKC活性和肌动蛋白聚合所致。

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