首页> 外文期刊>Acta Physiologiae Plantarum >Effects of carbon source, polyethylene glycol and abscisic acid on secondary embryo induction and maturation in rapeseed (Brassica napus L.) microspore-derived embryos
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Effects of carbon source, polyethylene glycol and abscisic acid on secondary embryo induction and maturation in rapeseed (Brassica napus L.) microspore-derived embryos

机译:碳源,聚乙二醇和脱落酸对油菜小孢子来源的胚次级胚诱导和成熟的影响

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In this study, the effects of carbon sources, abscisic acid (ABA) either alone or in combination with polyethylene glycol (PEG) were evaluated on secondary embryo (SE) induction and maturation in rapeseed microspore-derived embryos (MDE) of cultivars Global, PF704 and Option. Among various carbon sources tested (sucrose, glucose, fructose and sorbitol), the use of 0.3 M (300 mOsml−1) glucose and 0.2 M (200 mOsml−1) sorbitol in SE induction medium (for cultivars Global and PF704) and sorbitol at 0.2 and 0.3 M (200 and 300 mOsml−1, for cultivar Option), induced the highest secondary embryogenesis percentage (%SE). The highest number of SEs per each MDE (SE/MDE) was observed with 0.2 M (200 mOsml−1) sorbitol in cultivar Global and with 0.3 M (300 mOsml−1) glucose in cultivars PF704 and Option. In another part of this study, the effect of different concentrations of ABA (0, 20, 40, 60, 80 and 100 μM) and of a combined use of ABA (0 and 40 μM) and PEG 4000 or PEG 6000 at 15 g l−1 (3.75 and 2.5 mOsml−1, respectively) was examined on induction and maturation of SEs. In the first experiment, the use of ABA in SE induction medium reduced the mean number of SE/MDE in the three studied cultivars, whereas use of 40–80 μM ABA in SE induction medium increased the percentage of mature SEs in each cultivar. The combined use of PEG with or without ABA also reduced the mean number of SE/MDE compared with control, but resulted in significant enhancement of the percentages of mature SEs for the three cultivars.
机译:在这项研究中,评估了碳源,脱落酸(ABA)单独或与聚乙二醇(PEG)结合对油菜小孢子衍生胚(MDE)的次生胚(SE)诱导和成熟的影响, PF 704 和Option。在测试的各种碳源(蔗糖,葡萄糖,果糖和山梨糖醇)中,使用0.3 M(300 mOsml -1 )葡萄糖和0.2 M(200 mOsml -1 ) SE诱导培养基中的山梨糖醇(对于Global和PF 704 品种)和山梨糖醇在0.2和0.3 M(200和300 mOsml -1 ,对于品种选择)诱导最高次生胚胎发生率(%SE)。在全球品种中,山梨醇为0.2 M(200 mOsml -1 ),每MDE(SE / MDE)的SE数量最高,而0.3 M(300 mOsml -1 )的SE最高。 >)PF 704 和Option品种中的葡萄糖。在这项研究的另一部分中,不同浓度的ABA(0、20、40、60、80和100μM)以及ABA(0和40μM)与PEG 4000或PEG 6000在15 gl的联合使用的影响检测了 -1 (分别为3.75和2.5 mOsml -1 )对SEs的诱导和成熟。在第一个实验中,在SE诱导培养基中使用ABA降低了三个研究品种的SE / MDE平均数,而在SE诱导培养基中使用40–80μMABA增加了每个品种中成熟SE的百分比。与对照相比,有或没有ABA的PEG组合使用也降低了SE / MDE的平均数,但导致三个品种的成熟SEs百分比显着提高。

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