Changes in activity of phospholipase A2 (PLA2), a key enzyme in lipid metabolism and signal network in defence mechanisms, were investigated in Solanum species and Phytophthora infestans interaction. We have compared PLA2 activity in response to an elicitor, a culture filtrate (CF) derived from P. infestans, in non-host resistant Solanum nigrum var. gigantea, field resistant S. tuberosum cv Bzura and susceptible S. tuberosum clone H-8105. To elucidate the contribution of specific forms of PLA2 to plant defence mechanism reasonably selective PLA2 inhibitors, haloenol lactone suicide substrate (HELSS) and p-bromophenacyl bromide (BPB), which discriminate between Ca+2-independent PLA2 (iPLA2) and Ca+2-dependent secretory PLA2 (sPLA2), were used. The in vivo and in vitro effects of the inhibitors on PLA2 activity and on generation of reactive oxygen species (ROS) induced by CF in the studied plants were assayed. We found that PLA2 activity increased in response to CF treatment, displaying various kinetics and intensity depending on the resistance status of a given genotype. Differences among the genotypes in the effects of each inhibitor on CF-induced PLA2 activity and on ROS production may reflect the diversity of PLA2 isoforms in plants. Contrary to BPB, the inhibitory effect of HELSS was observable mainly on CF-induced PLA2 activity, which suggests that iPLA2 participates in signal transduction in defence reactions. Various effects of the two inhibitors on PLA2 activity and ROS production suggest different contribution of sPLA2 and iPLA2 to modulation of defence reactions in the interaction between Solanum genotypes and P. infestans.
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