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首页> 外文期刊>Acta Physiologiae Plantarum >Cost-effective in vitro conservation of banana using alternatives of gelling agent (isabgol) and carbon source (market sugar)
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Cost-effective in vitro conservation of banana using alternatives of gelling agent (isabgol) and carbon source (market sugar)

机译：使用胶凝剂（isabgol）和碳源（市场糖）的替代品，具有成本效益的体外香蕉保存

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摘要

To decrease the cost of in vitro conservation of banana cv. Karpura Chakkarakeli (AAB; Mysore subgroup) without any adverse effects on cultures, expensive components of medium such as sucrose and gelling agents, i.e. phytagel or agar (90% of the total cost of the medium), were replaced with inexpensive alternates such as market sugar and isabgol, respectively (Experiment 1). In general, no significant effects of isabgol and market sugar were observed on shoot (1.0–1.3 shoots/shoot explant) and root (1.5–2.0 roots/shoot explant) regeneration. Up to 12 months, 100% of cultures survived on isabgol-media, which was significantly higher than that on agar-media (79–83%) and on phytagel-media (51–57%). Isabgol-media with or without other constituents of medium were also tested for survival of banana cultures (Experiment 2); significant differences were observed for survival of cultures (20–100%). Slow growth of the cultures on isabgol-media was attributed to low availability of free water and consequently slower rate of transport of nutrients from isabgol matrix to the plantlets than that of other media tested, as evidenced by significantly lower relative matric potentials (0.801 and 0.804) of isabgol-media. In vitro conservation-derived plants grown in the field exhibited no significant morphological variations. The total cost of medium used for in vitro conservation of banana was decreased by 59% by using isabgol as an alternate gelling agent to agar and phytagel.
机译：为了降低香蕉简历的体外保存成本。 Karpura Chakkarakeli（AAB；迈索尔（Mysore）亚组）对培养物没有任何不良影响，培养基的昂贵成分（如蔗糖和胶凝剂，如phytagel或琼脂（占培养基总成本的90％））被廉价的替代品替代，如市场糖和伊沙糖醇（实验1）。通常，在芽（1.0–1.3芽/芽外植体）和根（1.5–2.0根/芽外植体）再生过程中，没有观察到异黄三醇和市售糖的显着影响。长达12个月，100％的培养物在isabgol培养基上存活，这显着高于琼脂培养基（79–83％）和植烷培养基（51–57％）。还测试了含或不含其他培养基成分的Isabgol培养基对香蕉培养物的存活率（实验2）；观察到培养物的存活率存在显着差异（20-100％）。依萨博格尔培养基上培养物的缓慢生长归因于游离水的利用率低，因此养分从依萨博格尔基质到小植株的转运速率比其他试验培养基慢，这是通过相对较低的基质电位（0.801和0.804）来证明的。 ）的isabgol-media。在田间生长的源自体外保存的植物没有表现出明显的形态变化。通过使用伊莎糖醇作为琼脂和phytagel的替代胶凝剂，用于香蕉体外保存的培养基的总成本降低了59％。

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