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首页> 外文期刊>Acta Physiologiae Plantarum >Differential gene expression analysis of a new Ogura CMS line and its maintainer in non-heading Chinese cabbage by cDNA-AFLP
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Differential gene expression analysis of a new Ogura CMS line and its maintainer in non-heading Chinese cabbage by cDNA-AFLP

机译：利用cDNA-AFLP分析一种新的Ogura CMS系及其保持子在非抽穗大白菜中的差异基因表达

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摘要

CMS is induced by the coordinated expression of certain mitochondrial and nuclear genes in flower development. Mitochondrial genes regulate manifestation of CMS, whereas nuclear genes regulate fertility phenotype and thus affect negatively. In this article, the buds of newly bred Ogura CMS of non-heading Chinese cabbage and its maintainer line as plant materials, genes differentially expressed transcripts were analyzed by cDNA-AFLP. Seventeen differently expressed genes were found in new Ogura CMS and nine genes in maintainer line. These genes were involved in energy metabolism, signal transduction, flower development, stress-related metabolism, transcription, etc. Expression patterns of three genes encoding BrCAM6, BrANK, BrTUB3 were verified by qRT-PCR in different organs and various stage flower buds of CMS and its maintainer line. The results revealed that two genes related to signal transduction, BrCAM6 and BrANK, were highly expressed in stamens and microspores of CMS than in maintainer line. As believed, these two genes involved in signal transduction of male sterile in CMS line. In comparison, BrTUB3 gene was accumulated in stamens and was expressed in significantly lower level in CMS line than in maintainer line. It expressed significantly lower in CMS than maintainer line after tetrad stage. This expression profile suggests that BrTUB3 played an important role in the development of the pollen, and may be closely related to male sterility.
机译：CMS是由某些线粒体和核基因在花发育中的协同表达诱导的。线粒体基因调节CMS的表现，而核基因调节生育力表型，从而产生负面影响。本文以非抽穗大白菜新近繁殖的Ogura CMS的芽及其维持系为植物材料，利用cDNA-AFLP技术分析了差异表达的转录本。在新的Ogura CMS中发现了17个不同表达的基因，在维持系中发现了9个基因。这些基因参与了能量代谢，信号转导，花朵发育，应激相关代谢，转录等。通过qRT-PCR验证了编码CMS，BrANK，BrTUB3的三种基因在CMS不同器官和不同阶段花蕾中的表达。及其维护者系列。结果表明，与信号转导相关的两个基因，BrCAM6和BrANK，在CMS的雄蕊和小孢子中比在维持系中高表达。如所相信的，这两个基因参与CMS系中雄性不育的信号转导。相比之下，BrTUB3基因在雄蕊中积累，并且在CMS系中的表达水平明显低于维持系。四分期后，它在CMS中的表达明显低于维持系。该表达概况表明BrTUB3在花粉的发育中起重要作用，并且可能与雄性不育密切相关。

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