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首页> 外文期刊>Acta Physiologiae Plantarum >Phenolic compounds accumulation in Hypericum ternum propagated in vitro and during plant development acclimatization
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Phenolic compounds accumulation in Hypericum ternum propagated in vitro and during plant development acclimatization

机译：贯叶连翘中酚类化合物的积累在体外和植物发育适应过程中传播

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摘要

The phenolic compound content of Hypericum ternum was investigated after micropropagation establishment and during acclimatization over the phenological development of the plant. Plantlets cultured in vitro on full Murashige and Skoog medium without growth regulators displayed higher phenolic compound yields, were acclimatized, and field grown. Production of total phenolic compounds as well as hyperoside, chlorogenic acid, quercitrin, guaijaverin, isoquercitrin, and uliginosin B were quantified at vegetative, flowering and fructification stages, and different plant organs (roots, stems, leaves and reproductive parts) showing that reproductive parts at flowering stage and the leaves at fructification stage were the main repository site of secondary metabolites, except for uliginosin B. The stems were the least accumulative organ, while the roots accumulated only hyperoside and uliginosin B. Moreover, the accumulation of most of the flavonoids and uliginosin B in acclimatized plants surpassed the levels found in the wild plant, warranting further research with the species.
机译：在建立微繁殖后和适应植物的物候发展过程中，研究了贯叶连翘的酚类化合物含量。在没有生长调节剂的完全Murashige和Skoog培养基上体外培养的小植株显示出更高的酚类化合物产量，使其适应环境并在田间生长。在营养，开花和果实化阶段以及不同植物器官（根，茎，叶和生殖部位）对总酚类化合物以及高脂苷，绿原酸，槲皮素，愈创甘油醚，异槲皮素和果胶素B的产生进行了定量，显示出生殖部位开花期和果实结实期的叶是次生代谢产物的主要贮藏位点，但芥子糖苷B除外。茎是最少的积累器官，而根部仅积聚多甙和芥子苷B。而且，大多数类黄酮的积累驯化植物中的芥子苷B超过了野生植物中发现的水平，因此有必要对该物种进行进一步研究。

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