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首页> 外文期刊>Acta Physiologiae Plantarum >Pleiotropic effect of flavonoid biosynthesis manipulation in transgenic potato plants
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Pleiotropic effect of flavonoid biosynthesis manipulation in transgenic potato plants

机译：黄酮类生物合成操纵对转基因马铃薯植物的多效性作用

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Three approaches were successfully used to manipulate content of flavonoids in transgenic plants. Overexpressing either the adaptor 14-3-3 protein or genes coding the key enzymes of the flavonoid biosynthesis pathway resulted in a significant increase in the compound content in potato tuber epidermis. The opposite effect was observed in transgenic plants in which these proteins were repressed; this strongly supports the view that the gene construct determines transgenic plant features. The most effective construct was, however, the one containing single dihydroflavonol reductase (DFR) gene in sense orientation. In all cases the increase in flavonoid content resulted in the expected enhancement of the antioxidant capacity of tuber extract. At the biochemical level a decrease in the starch content in transgenic plant overexpressing proteins regulating flavonoid biosynthesis was detected. In the case of glucosyl transferase (GT) gene overexpression, the content of phenolic compounds remained at the control level, however, the antioxidant capacity of tuber extracts significantly decreased. The GT plants grew faster and were more resistant to pathogen attacks, the tuber yield was significantly higher than that of nontransformants. Thus it is speculated that it is the chemical structure and degree of glucosylation of flavonoids rather than their quantity which determines transgenic plant features.
机译：成功地使用了三种方法来控制转基因植物中类黄酮的含量。过表达衔接子14-3-3蛋白或编码类黄酮生物合成途径关键酶的基因会导致马铃薯块茎表皮中化合物含量的显着增加。在转基因植物中观察到相反的效果，其中这些蛋白质被抑制。这有力地支持了基因构建体决定转基因植物特征的观点。然而，最有效的构建体是在正义方向上包含单个二氢黄酮醇还原酶（DFR）基因的构建体。在所有情况下，类黄酮含量的增加都会导致块茎提取物的抗氧化能力得到预期的提高。在生化水平上，检测到调节类黄酮生物合成的过表达植物的蛋白质中淀粉含量的减少。在葡萄糖基转移酶（GT）基因过表达的情况下，酚类化合物的含量保持在对照水平，但是，块茎提取物的抗氧化能力显着下降。 GT植物生长更快，对病原体的侵害更有抵抗力，块茎产量明显高于非转化株。因此推测，确定转基因植物特征的是类黄酮的化学结构和糖基化程度，而不是其数量。

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