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首页> 外文期刊>Advanced Functional Materials >Light-Controlled, High-Resolution Patterning of Living Engineered Bacteria Onto Textiles, Ceramics, and Plastic
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Light-Controlled, High-Resolution Patterning of Living Engineered Bacteria Onto Textiles, Ceramics, and Plastic

机译：在纺织品，陶瓷和塑料上对活体工程菌进行光控，高分辨率图案化

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Living cells can impart materials with advanced functions, such as sense and -respond, chemical production, toxin remediation, energy generation and storage, self-destruction, and self-healing. Here, an approach is presented to use light to pattern Escherichia coil onto diverse materials by controlling the expression of curli fibers that anchor the formation of a biofilm. Different colors oflight are used to express variants ofthe structural protein CsgA fused to different peptide tags. By projecting color images onto the material containing bacteria, this system can be used to pattern the growth of composite materials, including layers of protein and gold nanoparticles. This is used to pattern cells onto materials used for 3D printing, plastics (polystyrene), and textiles (cotton). Further, the adhered cells are demonstrated to respond to sensory information, including small molecules (IPTG and DAPG) and light from light-emitting diodes. This work advances the capacity to engineer responsive living materials in which cells provide diverse functionality.
机译：活细胞可以赋予材料以先进的功能，例如感知和响应，化学生产，毒素修复，能量产生和存储，自毁和自愈。在这里，提出了一种方法，通过控制锚定生物膜形成的卷曲纤维的表达，使用光将大肠杆菌线圈图案化到各种材料上。使用不同颜色的光来表达与不同肽标签融合的结构蛋白CsgA的变体。通过将彩色图像投射到含有细菌的材料上，该系统可用于图案化复合材料的生长，包括蛋白质和金纳米颗粒层。这用于在3D打印材料，塑料（聚苯乙烯）和纺织品（棉）上将单元图案化。此外，已证明粘附细胞对包括小分子（IPTG和DAPG）和来自发光二极管的光在内的感觉信息有反应。这项工作提高了工程响应能力的生活材料的能力，其中细胞提供了多种功能。

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