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首页> 外文期刊>Advanced Functional Materials >Directing Arrowhead Nanorod Dimers for MicroRNA In Situ Raman Detection in Living Cells
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Directing Arrowhead Nanorod Dimers for MicroRNA In Situ Raman Detection in Living Cells

机译：将箭头纳米棒二聚体引导为MicroRNA，在生物细胞中原位拉曼检测

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摘要

Directed self-assembled nanomaterials with biological applications have attracted great interest. Here, arrowhead gold nanorod (NR) dimers with side-by-side and end-to-end motifs (known as AHSBS and AHETE dimers, respectively) presented based on selective modification of arrowhead NRs with DNA and polyethylene glycol, possessing intense surface enhanced Raman scattering (SERS) signals, are assembled for in situ intracellular microRNA detection. The arrowhead NR dimers are capable of recognizing target microRNA in a sequence-specific manner as Raman signals significantly enhanced within dimers with both motifs. Following recognition, the dimers gradually disassemble, resulting in decreased SERS signals to achieve effective in situ Raman imaging and quantify the detection of target microRNA. The SERS intensity shows a linear relationship with intracellular target microRNA and a limit of detection of 0.011 amol ng(RNA)(-1) for the AHETE dimer and 0.023 amol ng(RNA)(-1) for the AHSBS dimer, respectively. The sensitivity for AHETE dimers is approximate to 2.1 times higher than that for AHSBS dimers, which is attributed to the small quantity of recognized molecules and stronger electrical enhancement, which causes greater SERS signals in the AHETE dimers. This approach opens up a new avenue for directed nanomaterials assembly and biological detection in living cells.
机译：具有生物应用的定向自组装纳米材料引起了极大的兴趣。这里，基于用DNA和聚乙二醇的箭头NRS选择性改性，具有并排的箭头金纳米棒（NR）二聚体（分别称为AHSB和AHETE二聚体），具有增强强烈的表面强烈的表面拉曼散射（SERS）信号，用于原位细胞内微瘤检测。箭头NR二聚体能够以序列特异性方式识别目标MicroRNA，因为拉曼信号显着增强了两个图案的二聚体。在识别之后，二聚体逐渐拆卸，导致SERS信号降低，以实现有效的原位拉曼成像，并量化靶微瘤的检测。 SERS强度显示与细胞内靶微度的线性关系和用于AHSBS二聚体的AHES二聚体的0.011AMOL NG（RNA）（ - 1）的检测限。 AHETE二聚体的灵敏度近似高于AHSB二聚体的2.1倍，这归因于少量识别的分子和更强的电增强，这导致AHETE二聚体中的更大的SERS信号。这种方法为导向纳米材料组装和生物检测开辟了一种新的养生细胞。

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  • 来源
    《Advanced Functional Materials》 |2020年第22期|2001451.1-2001451.10|共10页
  • 作者

    Li Chen; Li Si; Qu Aihua; Kuang Hua; Xu Liguang; Xu Chuanlai;

  • 作者单位

    Jiangnan Univ State Key Lab Food Sci & Technol Int Joint Res Lab Biointerface & Biodetect Sch Food Sci & Technol Wuxi 214122 Jiangsu Peoples R China;

    Jiangnan Univ State Key Lab Food Sci & Technol Int Joint Res Lab Biointerface & Biodetect Sch Food Sci & Technol Wuxi 214122 Jiangsu Peoples R China;

    Jiangnan Univ State Key Lab Food Sci & Technol Int Joint Res Lab Biointerface & Biodetect Sch Food Sci & Technol Wuxi 214122 Jiangsu Peoples R China;

    Jiangnan Univ State Key Lab Food Sci & Technol Int Joint Res Lab Biointerface & Biodetect Sch Food Sci & Technol Wuxi 214122 Jiangsu Peoples R China;

    Jiangnan Univ State Key Lab Food Sci & Technol Int Joint Res Lab Biointerface & Biodetect Sch Food Sci & Technol Wuxi 214122 Jiangsu Peoples R China;

    Jiangnan Univ State Key Lab Food Sci & Technol Int Joint Res Lab Biointerface & Biodetect Sch Food Sci & Technol Wuxi 214122 Jiangsu Peoples R China;

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  • 正文语种 eng
  • 中图分类
  • 关键词

    arrowhead nanorods; directed; Raman imaging; self-assembled nanomaterials; SERS;

    机译：箭头纳米棒;定向;拉曼成像;自组装的纳米材料;sers;

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