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首页> 外文期刊>Advanced Functional Materials >Dye-Sensitized Downconversion Nanoprobes with Emission Beyond 1500 nm for Ratiometric Visualization of Cancer Redox State
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Dye-Sensitized Downconversion Nanoprobes with Emission Beyond 1500 nm for Ratiometric Visualization of Cancer Redox State

机译：具有超过1500nm的发射的染料敏化下变化纳米侵子，用于癌症氧化还原状态的比率可视化

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摘要

Detection of glutathione (GSH) in the body is essential to accurately map the redox state of cells and real-time visualization of physiological and pathological conditions in vivo. However, traditional fluorescence (FL) imaging in the near-infrared I region (NIR-I, 650-900 nm) is difficult to quantitively visualize GSH in vivo due to the tissue autofluorescence background and disastrous photon scattering. Herein, a NIR-IIb (1500-1700 nm) nanoprobe consisting of 4-nitrophenol-Cy7 (NPh) conjugated lanthanide-based downconversion nanoparticles (DCNP@NPh-PEG) is developed for in vivo ratiometric imaging of GSH. In the presence of GSH, NPh shows responsively enhanced FL emission at 808 nm, thus enhancing FL signal at 1550 nm of DCNPs excited by 808 nm (F-1550,F- 808Ex) through non-radiative energy transfer (NRET) effect, while the fluorescence of DCNP at 1550 nm excited by 980 nm laser (F-1550,F- 980Ex) is stable because no NRET occurred. The ratiometric F-1550,F- 980Ex/F-1550,F- 808Ex value exhibits a linearship with GSH concentration ranged from 0-24 mm with detection limit of 0.3 mm. The NIR-IIb nanoprobe has excellent performance in detecting and imaging GSH in both subcutaneous tumor and orthotopic colon tumor in vivo with high accuracy and resolution. The design strategy of the ratiometric NIR-II FL nanoprobe based on the activated FERT effect provides a reliable tool for the development of NIR-II nanoprobes for accurate biosensing in vivo.
机译：在体内检测谷胱甘肽（GSH）对于准确地映射细胞的氧化还原状态和体内生理和病理条件的实时可视化至关重要。然而，由于组织自发荧光背景和灾难性光子散射，近红外I区域（NIR-I，650-900nm）中的传统荧光（FL）成像难以定量地在体内可视化GSH。在此，由4-硝基苯酚-CY7（NPH）缀合的镧系元素的下转化纳米颗粒（DCNP @ NPH-PEG）组成的NIR-IIB（1500-1700nm）纳米骨（DCNP @ NPH-PEG）用于GSH的体内比例。在GSH的存在下，NPH显示808nm处的响应性增强，从而通过非辐射能量转移（NRET）效应，在808nm（f-1550，f-808ex）中激发的1550nm的1550nm的550nm的F1信号。通过980nm激光（F-1550，F-980Ex）激发的1550nm的DCNP荧光是稳定的，因为没有发生nRET。比率F-1550，F-980EX / F-1550，F-808ex值表现出具有GSH浓度的线性序列，范围为0-24毫米，检测限为0.3mm。 NIR-IIB Nanoprobe在体内检测和成像在皮下肿瘤和原位结肠肿瘤中具有高精度和分辨率的优异性能。基于活性的动物效应的比率NIR-II FL NANOPORBOBE的设计策略为NIR-II纳米素的开发提供了可靠的工具，用于高温体内生物溶解。

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  • 来源
    《Advanced Functional Materials》 |2021年第16期|2009942.1-2009942.11|共11页
  • 作者

    Wang Chenlu; Lin Hongxin; Ge Xiaoguang; Mu Jing; Su Lichao; Zhang Xuan; Niu Meng; Yang Huanghao; Song Jibin;

  • 作者单位

    Fuzhou Univ Coll Chem MOE Key Lab Analyt Sci Food Safety & Biol Fuzhou 350108 Peoples R China;

    Fujian Normal Univ Fujian Prov Key Lab Photon Technol Minist Educ Key Lab Optoelect Sci & Technol Med Fuzhou 350007 Peoples R China;

    Fuzhou Univ Coll Chem MOE Key Lab Analyt Sci Food Safety & Biol Fuzhou 350108 Peoples R China;

    Peking Univ Inst Precis Med Shenzhen Hosp Shenzhen 518036 Peoples R China;

    Fuzhou Univ Coll Chem MOE Key Lab Analyt Sci Food Safety & Biol Fuzhou 350108 Peoples R China;

    Fuzhou Univ Coll Chem MOE Key Lab Analyt Sci Food Safety & Biol Fuzhou 350108 Peoples R China;

    Fuzhou Univ Coll Chem MOE Key Lab Analyt Sci Food Safety & Biol Fuzhou 350108 Peoples R China;

    Fuzhou Univ Coll Chem MOE Key Lab Analyt Sci Food Safety & Biol Fuzhou 350108 Peoples R China;

    Fuzhou Univ Coll Chem MOE Key Lab Analyt Sci Food Safety & Biol Fuzhou 350108 Peoples R China;

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  • 关键词

    bioimaging; glutathione; nanoprobes; NIR#8208; II; ratiometric fluorescence;

    机译：生物体;谷胱甘肽;纳米诡脂;德里＆＃8208;II;比例荧光;

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