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首页> 外文期刊>Advanced Functional Materials >Integrated Fluorescent Nanoprobe Design for High-Speed In Vivo Two-Photon Microscopic Imaging of Deep-Brain Vasculature in Mice
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Integrated Fluorescent Nanoprobe Design for High-Speed In Vivo Two-Photon Microscopic Imaging of Deep-Brain Vasculature in Mice

机译：集成荧光纳米孔设计，用于小鼠深脑脉管系统的体内二光子微观成像的高速

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摘要

High-speed two-photon microscopy can be used to analyze vascular dynamics in living animals and is essential for the understanding of brain diseases. Recent advances in fluorescent probes/optical systems have allowed successful imaging of the hippocampal vasculature in the deep brain of mice (1 mm from the brain surface) under low-speed conditions (1-2 fps); however, using high-speed techniques (30 fps), observation of the deep-brain vasculature is still challenging. Here, a new nanoemulsion that encapsulates thousands of red-emissive pyrene dye molecules while maintaining their high two-photon brightness [1.5 x 10(2) GM (GM = 10(-50) cm(4)center dot s center dot photon(-1)center dot molecule(-1)) at 960 nm excitation] and delivers a large amount of such pyrene dyes (65 nmol) into the blood vessels of mice is developed. Remarkably, the nanoprobe is found to exploit the inherent performance of a commonly used Ti:sapphire excitation laser and a sensitive gallium arsenide phosphide nondescanned fluorescence detector to the limit, enabling visualization of the brain vasculature under the cortex region of mice (up to 1.5 mm) under very low-speed conditions. As a highlight, such a nanoprobe is successfully used to directly observe the blood flow in the hippocampal CA1 region (1.1 mm) through high-speed resonant scanning (120 fps).
机译：高速双光子显微镜可用于分析活动物中的血管动态，对脑病的理解至关重要。荧光探针/光学系统的最近进步允许在低速条件下（1-2 FPS）在小鼠的深脑（距大脑表面1mm）的深脑中成功成功成功;然而，使用高速技术（＆ 30 fps），观察深脑脉管系统仍然具有挑战性。这里，一种新的纳米乳液，其封装成千上万的红发酵丁烯染料分子，同时保持其高双光子亮度[1.5×10（2）克（GM = 10（-50）cm（4）中心点S中心点光子（ -1）中央点分子（-1））在960nm激发中，发出大量这样的这种芘染料（65nmol）在小鼠的血管中发出。值得注意的是，纳米孔发现常用的Ti的固有性能：蓝宝石激光激光器和敏感镓砷化镓磷化磷脂非荧光荧光探测器的极限，使得在小鼠的皮质区域下的脑脉管系统可视化（高达1.5mm ）在非常低速的条件下。作为亮点，通过高速共振扫描（120fps）成功地使用这种纳米骨瓣直接观察海马CA1区（1.1mm）中的血流。

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  • 来源
    《Advanced Functional Materials》 |2021年第20期|2010698.1-2010698.9|共9页
  • 作者

    Takezaki Minami; Kawakami Ryosuke; Onishi Shozo; Suzuki Yasutaka; Kawamata Jun; Imamura Takeshi; Hadano Shingo; Watanabe Shigeru; Niko Yosuke;

  • 作者单位

    Kochi Univ Interdisciplinary Sci Unit Multidisciplinary Sci Cluster Res & Educ Fac 2-5-1 Akebono Cho Kochi Kochi 7808520 Japan;

    Ehime Univ Grad Sch Med Dept Mol Med Pathogenesis Toon Ehime 7910295 Japan;

    Yamaguchi Univ Grad Sch Sci & Technol Innovat 1677-1 Yoshida Yamaguchi Yamaguchi 7538512 Japan;

    Yamaguchi Univ Grad Sch Sci & Technol Innovat 1677-1 Yoshida Yamaguchi Yamaguchi 7538512 Japan;

    Yamaguchi Univ Grad Sch Sci & Technol Innovat 1677-1 Yoshida Yamaguchi Yamaguchi 7538512 Japan;

    Ehime Univ Grad Sch Med Dept Mol Med Pathogenesis Toon Ehime 7910295 Japan;

    Kochi Univ Interdisciplinary Sci Unit Multidisciplinary Sci Cluster Res & Educ Fac 2-5-1 Akebono Cho Kochi Kochi 7808520 Japan;

    Kochi Univ Interdisciplinary Sci Unit Multidisciplinary Sci Cluster Res & Educ Fac 2-5-1 Akebono Cho Kochi Kochi 7808520 Japan;

    Kochi Univ Interdisciplinary Sci Unit Multidisciplinary Sci Cluster Res & Educ Fac 2-5-1 Akebono Cho Kochi Kochi 7808520 Japan;

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  • 关键词

    brain vasculature imaging; dye#8208; loaded nanoemulsions; fluorescent nanoprobes; high#8208; speed imaging; two#8208; photon microscopy;

    机译：脑血管系统成像;染料＆＃8208;装载纳米乳液;荧光纳米体;高＆＃8208;速度成像;两个＆＃8208;光子显微镜;

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