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首页> 外文期刊>Advanced Functional Materials >A Novel Hydrogel Surface Grafted With Dual Functional Peptides for Sustaining Long-Term Self-Renewal of Human Induced Pluripotent Stem Cells and Manipulating Their Osteoblastic Maturation
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A Novel Hydrogel Surface Grafted With Dual Functional Peptides for Sustaining Long-Term Self-Renewal of Human Induced Pluripotent Stem Cells and Manipulating Their Osteoblastic Maturation

机译：一种新型的双功能肽接枝的水凝胶表面，用于维持人类诱导的多能干细胞的长期自我更新并操纵其成骨细胞的成熟。

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摘要

Realizing the clinical potential of human induced pluripotent stem cells (hiPSCs) in bone regenerative medicine requires the development of safe and chemically defined biomaterials for expansion of hiPSCs followed by directing their lineage commitment to osteoblasts. In this study, novel multipurpose peptide-presenting hydrogel surfaces are prepared on common tissue culture plates via carboxymethyl chitosan grafting and subsequent immobilization of two functional peptides allowing for in vitro feeder-free culture, long-term self-renewal, and osteogenic induction of hiPSCs. After vitronectin (VN) peptide modification, the engineered surfaces facilitate adhesion, proliferation, colony formation, and the maintenance of pluripotency of hiPSCs up to passage 10 under fully defined conditions without Matrigel or protein coating. Further, this synthetic niche exhibits an appealing regulatory effect on the osteogenic conversion of hiPSCs to osteoblastic phenotype without an embryoid body formation step by co-decoration of different ratios of VN and bone-forming peptide. Such a well-defined, xeno-free 2D engineered microenvironment not only helps to accelerate the clinical development of hiPSCs, but also provides a safe and robust platform for the generation of osteoblast-like cells or bone-like tissues at different maturation levels. Thus, the strategy may hold great potential for application in cell therapy and bone tissue engineering.
机译：要在骨再生医学中实现人类诱导的多能干细胞（hiPSC）的临床潜力，就需要开发安全且化学定义明确的生物材料来扩展hiPSC，然后将其谱系定向到成骨细胞上。在这项研究中，通过羧甲基壳聚糖接枝并随后将两种功能性肽固定在普通的组织培养板上，制备了新颖的多功能肽呈递水凝胶表面，从而实现了无饲养层的体外培养，长期自我更新和成骨诱导性hiPSCs 。经过玻连蛋白（VN）肽修饰后，在没有基质胶或蛋白质包衣的完全限定条件下，经过工程改造的表面可促进黏附，增殖，集落形成以及hiPSC的多能性维持直至第10代。此外，该合成的小生境对hiPSC的成骨转化向成骨细胞表型表现出有吸引力的调节作用，而无需通过共装饰不同比例的VN和成骨肽来形成胚状体形成步骤。这种定义明确，无异种2D工程的微环境不仅有助于加速hiPSC的临床发展，而且还为在不同成熟水平下生成成骨细胞样细胞或骨样组织提供了安全可靠的平台。因此，该策略可能在细胞治疗和骨组织工程中具有巨大的应用潜力。

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  • 来源
    《Advanced Functional Materials》 |2018年第11期|1705546.1-1705546.17|共17页
  • 作者

    Deng Yi; Wei Shicheng; Yang Lei; Yang Weizhong; Dargusch Matthew S.; Chen Zhi-Gang;

  • 作者单位

    Peking Univ, Acad Adv Interdisciplinary Studies, Natl Engn Lab Digital & Mat Technol Stomatol, Sch & Hosp Stomatol,Cent Lab, Beijing 100081, Peoples R China;

    Peking Univ, Acad Adv Interdisciplinary Studies, Natl Engn Lab Digital & Mat Technol Stomatol, Sch & Hosp Stomatol,Cent Lab, Beijing 100081, Peoples R China;

    Sichuan Univ, Sch Mat Sci & Engn, Sch Chem Engn, Chengdu 610065, Sichuan, Peoples R China;

    Sichuan Univ, Sch Mat Sci & Engn, Sch Chem Engn, Chengdu 610065, Sichuan, Peoples R China;

    Univ Queensland, Mat Engn, Brisbane, Qld 4072, Australia;

    Univ Queensland, Mat Engn, Brisbane, Qld 4072, Australia;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    engineered surfaces; expansion; functional peptides; hiPSCs; osteoblastic maturation;

    机译：工程表面;扩展;功能肽;hiPSCs;成骨细胞成熟;

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