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首页> 外文期刊>Advanced Functional Materials >A Universal and Ultrastable Mineralization Coating Bioinspired from Biofilms
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A Universal and Ultrastable Mineralization Coating Bioinspired from Biofilms

机译：从生物膜获得生物启发的通用且超稳定的矿化涂层

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摘要

A simple and universal method for manufacturing a mineralization coating on various surfaces is developed using a biofilm-based material obtained from engineered curli nanofibers. The amyloid protein (CsgA) is the main proteinaceous component in the Escherichia coli (E. coli) biofilm, which can withstand detergents in the harsh environment. The peptide sequence DDDEEK is bioinspired from salivary acquired pellicles in the dental plaque biofilm, having a strong ability to absorb mineral ions and induce the formation of biominerals. The bioinspired coating is successfully secreted by the engineered E. coli, which is transformed with a recombinant plasmid for expression with T7 promoter (PET), namely PET-22b-CsgA-DDDEEK plasmid. The uniform coating can bear shear force and stay on virtually any type of material surface for at least one month. Moreover, the coated slices had a good mineralization performance and better stability than hydroxyapatite (HA)-spray slices. Furthermore, MG63 cells on the bioactive HA layer induced by the coating possess a better growth capacity than those on the commercial product Matrigel. The animal experiment results suggest that the coated Ti6Al4V screws with induced HA present better osteogenicity and osseointegration than HA-sprayed screws after 12 weeks, as well as no extra immunogenicity. Thus, the coating is highly promising for biomedical applications.%1802730.1-1802730.12
机译：使用从工程卷曲纳米纤维获得的生物膜基材料，开发了一种用于在各种表面上制造矿化涂层的简单通用方法。淀粉样蛋白（CsgA）是大肠杆菌（E. coli）生物膜中的主要蛋白质成分，可以在恶劣的环境中耐受洗涤剂。 DDDEEK肽序列是由牙菌斑生物膜中唾液获得的薄膜提供生物启发而来的，具有很强的吸收矿物质离子和诱导生物矿物质形成的能力。生物启发的涂层成功地被工程化的大肠杆菌分泌，然后用重组质粒转化以与T7启动子（PET）表达，即PET-22b-CsgA-DDDEEK质粒。均匀的涂层可以承受剪切力，并且实际上可以在任何类型的材料表面上停留至少一个月。此外，与羟基磷灰石（HA）喷涂片相比，涂层片具有良好的矿化性能和更好的稳定性。此外，由涂层诱导的生物活性HA层上的MG63细胞比市售产品Matrigel上的MG63细胞具有更好的生长能力。动物实验结果表明，与HA喷涂的螺钉相比，带有HA的涂层Ti6Al4V螺钉在12周后具有更好的成骨性和骨整合性，并且没有额外的免疫原性。因此，该涂层对于生物医学应用是非常有前途的。％1802730.1-1802730.12

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  • 来源
    《Advanced Functional Materials》 |2018年第32期|1802730.1-1802730.12|共12页
  • 作者

    Yang Xiao; Li Zhenhua; Xiao Hong; Wang Ning; Li Yanpu; Xu Xinyuan; Chen Zhijun; Tan Hong; Li Jianshu;

  • 作者单位

    Sichuan Univ, Coll Polymer Sci & Engn, State Key Lab Polymer Mat Engn, 24 South Sect 1 Yinhuan Rd, Chengdu 610065, Sichuan, Peoples R China;

    Jilin Univ, Inst Theoret Chem, State Key Lab Supramol Struct & Mat, 2699 Qianjin St, Changchun 130012, Jilin, Peoples R China;

    Sichuan Univ, West China Hosp, Dept Pain Management, 37 GuoXue Xiang, Chengdu 610041, Sichuan, Peoples R China;

    Sichuan Univ, West China Hosp, Regenerat Med Res Ctr, 37 GuoXue Xiang, Chengdu 61004, Sichuan, Peoples R China;

    Sichuan Univ, Coll Polymer Sci & Engn, State Key Lab Polymer Mat Engn, 24 South Sect 1 Yinhuan Rd, Chengdu 610065, Sichuan, Peoples R China;

    Sichuan Univ, Coll Polymer Sci & Engn, State Key Lab Polymer Mat Engn, 24 South Sect 1 Yinhuan Rd, Chengdu 610065, Sichuan, Peoples R China;

    Jilin Univ, Inst Theoret Chem, State Key Lab Supramol Struct & Mat, 2699 Qianjin St, Changchun 130012, Jilin, Peoples R China;

    Sichuan Univ, Coll Polymer Sci & Engn, State Key Lab Polymer Mat Engn, 24 South Sect 1 Yinhuan Rd, Chengdu 610065, Sichuan, Peoples R China;

    Sichuan Univ, Coll Polymer Sci & Engn, State Key Lab Polymer Mat Engn, 24 South Sect 1 Yinhuan Rd, Chengdu 610065, Sichuan, Peoples R China;

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  • 正文语种 eng
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  • 关键词

    bioinspired materials; biomineralization; functional coatings; nanofibers; peptides;

    机译：生物启发的材料;生物矿化;功能涂层;纳米纤维;肽;

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