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A Protein-Nanocellulose Paper for Sensing Copper Ions at the Nano- to Micromolar Level

机译:一种蛋白质-纳米纤维素纸,用于在纳摩尔至微摩尔水平检测铜离子

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摘要

Tracing heavy metals is a crucial issue in both environmental and medical samples. In this work, a sensing biomolecule, the cyanobacterial C-phycocyanin (CPC), is integrated into a nanocellulose matrix, and with this, a biosensor for copper ions is developed. The assembly of CPC-functionalized nanocellulose into a red-fluorescent, copper-sensitive hybrid film CySense, enhances protein stability and facilitates the reuse and the regeneration of the sensor for several cycles over 7 days. CySense is suitable for the analysis of complex medical samples such as human serum filtrate. The reported biosensor reliably detects copper ion contents with a lower detection limit of 200 x 10(-9)m and an IC50 of 4.9 x 10(-6)m as changes in fluorescence emission intensity that can be measured with a fluorimeter or a microarray laser scanner.
机译:在环境和医学样品中,重金属的追踪都是至关重要的问题。在这项工作中,将传感生物分子即蓝细菌C-藻蓝蛋白(CPC)整合到纳米纤维素基质中,并以此开发了一种铜离子生物传感器。将CPC功能化的纳米纤维素组装成对荧光敏感的铜红色杂化薄膜CySense,可增强蛋白质稳定性,并在7天的多个循环中促进传感器的重复使用和再生。 CySense适用于分析复杂的医学样品,例如人血清滤液。报道的生物传感器能够可靠地检测出铜离子含量,其下限为200 x 10(-9)m,IC50为4.9 x 10(-6)m,可以通过荧光计或微阵列测量荧光强度的变化激光扫描仪。

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