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Harvesting of Living Cell Sheets by the Dynamic Generation of Diffractive Photothermal Pattern on PEDOT

机译:通过在PEDOT上动态生成衍射光热图来收集活细胞片

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Near infrared (NIR) photothermal pattern on conductive polymer film enables unique approaches to harvest large-area cell sheets with various patterns without the use of patterned culture dish. The NIR photothermal pattern is generated from a patterned optical lens (POL), which creates a dynamic near IR light pattern and the corresponding photothermal pattern (PTP) on the polymer film. The POL is prepared from transparent polydimethylsiloxane designed to generate various light patterns. The PTPs allow a noninvasive harvest of cultured cells as an intact living cell sheet with a high harvesting efficiency ((cell) > 100). Various PTPs are generated by the diffraction of NIR light through POLs having different micropatterns, which afford cell sheets with a desired pattern without changing the original cell morphology at cultured state. Furthermore, a large-area living cell sheet is obtained with a detached area larger than 19 cm(2), which is the largest living cell sheet up to date. Further optical engineering of the harvesting system allows multiple productions of cell sheets with one dose of light. It is possible to harvest cell sheets not only from human fibroblast cells but also from human adipose-derived stem cells, indicating that the method can be applied to engineer various cells.
机译:导电聚合物膜上的近红外(NIR)光热图案使采用独特的方法可以收获具有各种图案的大面积细胞片,而无需使用带图案的培养皿。 NIR光热图案由图案化的光学透镜(POL)生成,该透镜在聚合物薄膜上产生动态的近红外光图案和相应的光热图案(PTP)。 POL是由透明的聚二甲基硅氧烷制成的,该透明的聚二甲基硅氧烷旨在产生各种光图案。 PTP允许以高收获效率((cell)> 100)作为完整的活细胞片无创地收获培养细胞。通过具有不同微图案的POL通过NIR光的衍射产生各种PTP,这使细胞片具有所需的图案,而不会改变培养状态下的原始细胞形态。此外,获得的大面积活细胞片的分离面积大于19 cm(2),这是迄今为止最大的活细胞片。采集系统的进一步光学工程技术可以用一剂光多次生产细胞片。不仅可以从人成纤维细胞中收获细胞片,而且可以从人脂肪来源的干细胞中收获细胞片,这表明该方法可以用于工程化各种细胞。

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