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首页> 外文期刊>Advanced Functional Materials >Screening of Nanocomposite Scaffolds Arrays Using Superhydrophobic-Wettable Micropatterns
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Screening of Nanocomposite Scaffolds Arrays Using Superhydrophobic-Wettable Micropatterns

机译：使用超疏水可湿式微模式筛选纳米复合支架阵列。

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摘要

Platforms containing multiple arrays for high-throughput screening are demanded in the development of biomaterial libraries. Here, an array platform for the combinatorial analysis of cellular interactions and 3D porous biomaterials is described. Using a novel method based on computer-aided manufacturing, wettable regions are printed on superhydrophobic surfaces, generating isolated spots. This freestanding benchtop array is used as a tool to deposit naturally derived polymers, chitosan and hyaluronic acid, with bioactive glass nanoparticles (BGNPs) to obtain a scaffold matrix. The effect of fibronectin adsorption on the scaffolds is also tested. The biomimetic nanocomposite scaffolds are shown to be osteoconductive, non-cytotoxic, promote cell adhesion, and regulate osteogenic commitment. The method proves to be suitable for screening of biomaterials in 3D cell cultures as it can recreate a multitude of combinations on a single platform and identify the optimal composition that drives to desired cell responses. The platforms are fully compatible with commercially routine cell culture labware and established characterization methods, allowing for a standard control and easy adaptability to the cell culture environment. This study shows the value of 3D structured array platforms to decode the combinatorial interactions at play in cell microenvironments.
机译：生物材料文库的开发需要包含用于高通量筛选的多个阵列的平台。在此，描述了用于细胞相互作用和3D多孔生物材料的组合分析的阵列平台。使用基于计算机辅助制造的新颖方法，将可湿性区域印刷在超疏水性表面上，产生孤立的斑点。这种独立式台式阵列用作将天然衍生的聚合物，壳聚糖和透明质酸与生物活性玻璃纳米粒子（BGNP）沉积在一起以获得支架基质的工具。还测试了纤连蛋白吸附在支架上的作用。仿生纳米复合材料支架显示出骨传导性，无细胞毒性，促进细胞粘附并调节成骨作用。该方法被证明适合筛选3D细胞培养物中的生物材料，因为它可以在单个平台上重新创建多种组合，并确定驱动所需细胞反应的最佳成分。该平台与商业常规细胞培养实验室器具和已建立的表征方法完全兼容，可实现标准控制并易于适应细胞培养环境。这项研究显示了3D结构化阵列平台对在细胞微环境中发挥作用的组合相互作用进行解码的价值。

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  • 来源
    《Advanced Functional Materials》 |2017年第28期|1701219.1-1701219.12|共12页
  • 作者

    Leite Alvaro J.; Oliveira Mariana B.; Caridade Sofia G.; Mano Joao F.;

  • 作者单位

    Univ Minho, Res Grp Biomat Biodegradables & Biomimet 3Bs, Avepk Parque Ciencia & Tecnol, P-4805017 Barco, Guimaraes, Portugal|European Inst Excellence Tissue Engn & Regenerat, Avepk Parque Ciencia & Tecnol, P-4805017 Barco, Guimaraes, Portugal|ICVS 3Bs PT Govt Associate Lab, P-4805017 Guimaraes, Portugal|Univ Aveiro, Dept Chem, CICECO, P-3810193 Aveiro, Portugal;

    Univ Minho, Res Grp Biomat Biodegradables & Biomimet 3Bs, Avepk Parque Ciencia & Tecnol, P-4805017 Barco, Guimaraes, Portugal|European Inst Excellence Tissue Engn & Regenerat, Avepk Parque Ciencia & Tecnol, P-4805017 Barco, Guimaraes, Portugal|ICVS 3Bs PT Govt Associate Lab, P-4805017 Guimaraes, Portugal;

    Univ Minho, Res Grp Biomat Biodegradables & Biomimet 3Bs, Avepk Parque Ciencia & Tecnol, P-4805017 Barco, Guimaraes, Portugal|European Inst Excellence Tissue Engn & Regenerat, Avepk Parque Ciencia & Tecnol, P-4805017 Barco, Guimaraes, Portugal|ICVS 3Bs PT Govt Associate Lab, P-4805017 Guimaraes, Portugal|Univ Aveiro, Dept Chem, CICECO, P-3810193 Aveiro, Portugal;

    Univ Minho, Res Grp Biomat Biodegradables & Biomimet 3Bs, Avepk Parque Ciencia & Tecnol, P-4805017 Barco, Guimaraes, Portugal|European Inst Excellence Tissue Engn & Regenerat, Avepk Parque Ciencia & Tecnol, P-4805017 Barco, Guimaraes, Portugal|ICVS 3Bs PT Govt Associate Lab, P-4805017 Guimaraes, Portugal|Univ Aveiro, Dept Chem, CICECO, P-3810193 Aveiro, Portugal;

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  • 正文语种 eng
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  • 关键词

    array-based screening; combinatorial biomaterials screening; flat platforms; high-throughput screening; nanocomposites; nondestructive image analysis;

    机译：基于阵列的筛选;组合生物材料筛选;平台;高通量筛选;纳米复合材料;无损图像分析;

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