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首页> 外文期刊>Advanced Functional Materials >MRI/SPECT/Fluorescent Tri-Modal Probe for Evaluating the Homing and Therapeutic Efficacy of Transplanted Mesenchymal Stem Cells in a Rat Ischemic Stroke Model
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MRI/SPECT/Fluorescent Tri-Modal Probe for Evaluating the Homing and Therapeutic Efficacy of Transplanted Mesenchymal Stem Cells in a Rat Ischemic Stroke Model

机译：MRI / SPECT /荧光三模态探针评估大鼠缺血性中风模型中移植的间充质干细胞的归巢和治疗功效

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摘要

Quantitatively tracking engraftment of intracerebrally or intravenously transplanted stem cells and evaluating their concomitant therapeutic efficacy for stroke has been a challenge in the field of stem cell therapy. In this study, first, an MRI/SPECT/fluorescent tri-modal probe (~(125)I-fSiO4@SPIOs) is synthesized for quantitatively tracking mesenchymal stem cells (MSCs) transplanted intracerebrally or intravenously into stroke rats, and then the therapeutic efficacy of MSCs delivered by both routes and the possible mechanism of the therapy are evaluated. It is demonstrated that ~((125))I-fSiO4@SPIOs have high efficiency for labeling MSCs without affecting their viability, differentiation, and proliferation capacity, and found that 35% of intracerebrally injected MSCs migrate along the corpus callosum to the lesion area, while 90% of intravenously injected MSCs remain trapped in the lung at 14 days after MSC transplantation. However, neurobehavioral outcomes are significantly improved in both transplantation groups, which are accompanied by increases of vascular endothelial growth factor, basic fibroblast growth factor, and tissue inhibitor of metalloproteinases-3 in blood, lung, and brain tissue (ρ ＜ 0.05). The study demonstrates that ~(125)I-fSiO4@SPIOs are robust probe for long-term tracking of MSCs in the treatment of ischemic brain and MSCs delivered via both routes improve neurobehavioral outcomes in ischemic rats.
机译：定量追踪脑内或静脉内移植的干细胞的植入并评估其对中风的治疗效果一直是干细胞治疗领域的挑战。在这项研究中，首先，合成MRI / SPECT /荧光三峰探针（〜（125）I-fSiO4 @ SPIOs），以定量追踪经脑或静脉内移植入中风大鼠的间充质干细胞（MSC），然后进行治疗评价了通过两种途径递送的MSC的功效以及该治疗的可能机制。结果表明，〜（（125））I-fSiO4 @ SPIOs在不影响其生存能力，分化和增殖能力的情况下，对MSCs的标记效率很高，并且发现35％的脑内注射MSCs沿着call体迁移到病变区域。 ，而MSC移植后第14天，仍有90％的静脉注射MSC被困在肺中。但是，两个移植组的神经行为结果均得到显着改善，并伴有血管内皮生长因子，碱性成纤维细胞生长因子以及血液，肺和脑组织中金属蛋白酶-3的组织抑制剂的增加（ρ＜0.05）。该研究表明，〜（125）I-fSiO4 @ SPIOs是长期跟踪治疗缺血性脑的MSC的有力探针，通过这两种途径递送的MSC均可改善缺血大鼠的神经行为结果。

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  • 来源
    《Advanced Functional Materials》 |2015年第7期|1024-1034|共11页
  • 作者

    Yaohui Tang; Chunfu Zhang; Jixian Wang; Xiaojie Lin; Lu Zhang; Yi Yang; Yongting Wang; Zhijun Zhang; Jeff W. M. Bulte; Guo-Yuan Yang;

  • 作者单位

    State Key Laboratory of Oncogenes and Related Genes School of Biomedical Engineering Shanghai Jiao Tong University Shanghai 200030, China;

    State Key Laboratory of Oncogenes and Related Genes School of Biomedical Engineering Shanghai Jiao Tong University Shanghai 200030, China,School of Biomedical Engineering and Med-X Research Institute Shanghai Jiao Tong University Shanghai 200030, China;

    Department of Neurology, Shanghai Ruijin Hospital School of Medicine Shanghai Jiao Tong University Shanghai 200030, China;

    School of Biomedical Engineering and Med-X Research Institute Shanghai Jiao Tong University Shanghai 200030, China;

    School of Biomedical Engineering and Med-X Research Institute Shanghai Jiao Tong University Shanghai 200030, China;

    School of Biomedical Engineering and Med-X Research Institute Shanghai Jiao Tong University Shanghai 200030, China;

    School of Biomedical Engineering and Med-X Research Institute Shanghai Jiao Tong University Shanghai 200030, China;

    School of Biomedical Engineering and Med-X Research Institute Shanghai Jiao Tong University Shanghai 200030, China;

    Russell H. Morgan Department of Radiology and Radiological Science Division of MR Research Cellular Imaging Section, Institute for Cell Engineering The Johns Hopkins University School of Medicine Baltimore, MD 21205, USA;

    School of Biomedical Engineering and Med-X Research Institute Shanghai Jiao Tong University Shanghai 200030, China,Department of Neurology, Shanghai Ruijin Hospital School of Medicine Shanghai Jiao Tong University Shanghai 200030, China;

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