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Electrochemical Quantification of Escherichia coli with DNA Nanostructure

机译:具有DNA纳米结构的大肠杆菌的电化学定量

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摘要

Escherichia coli is an indicator of fecal contamination in recreational water and some strains are responsible for serious food poisoning in humans. Therefore, having a rapid, sensitive, selective, and simple E. coli detection is important. In this study, DNA nanopyramids anchor is utilized for immunological detection of E. coli lipopolysaccharides, lysate, and whole bacteria. By measuring the E. coli amount electrochemically, detection limits of 0.20 ng mL(-1)E. coli lipopolysaccharides and 1.20 CFU mL(-1) equivalent of lysed E. coli bacteria are obtained. Our method confers selectivity to detect E. coli in the presence of various other macromolecules. Therefore, our system can be developed to test the E. coli content in real water samples.
机译:大肠杆菌是休闲用水中粪便污染的指标,某些菌株导致人类严重食物中毒。因此,快速,灵敏,选择性和简单的大肠杆菌检测非常重要。在这项研究中,DNA纳米金字塔锚被用于免疫检测大肠杆菌脂多糖,裂解物和整个细菌。通过电化学测量大肠杆菌量,检出限为0.20 ng mL(-1)E。大肠杆菌脂多糖和1.20 CFU mL(-1)当量的裂解大肠杆菌。我们的方法赋予在多种其他大分子存在下检测大肠杆菌的选择性。因此,可以开发我们的系统来测试实际水样中的大肠杆菌含量。

著录项

  • 来源
    《Advanced Functional Materials》 |2015年第25期|3840-3846|共7页
  • 作者单位

    Natl Univ Singapore, Dept Chem & Biomol Engn, Singapore 117585, Singapore;

    Natl Univ Singapore, Dept Chem & Biomol Engn, Singapore 117585, Singapore;

    Natl Univ Singapore, Dept Chem & Biomol Engn, Singapore 117585, Singapore;

    Natl Univ Singapore, Dept Chem & Biomol Engn, Singapore 117585, Singapore;

    Natl Univ Singapore, Natl Univ Hosp, Natl Univ Hlth Syst, Dept Surg,Yong Loo Lin Sch Med,Emergency Med Dept, Singapore 119074, Singapore;

    Natl Univ Singapore, Dept Chem & Biomol Engn, Singapore 117585, Singapore;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    biosensor; DNA nanostructures; Escherichia coli;

    机译:生物传感器DNA纳米结构大肠杆菌;

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