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首页> 外文期刊>Advanced Functional Materials >Vectorially Imprinted Hybrid Nanofilm for Acetylcholinesterase Recognition
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Vectorially Imprinted Hybrid Nanofilm for Acetylcholinesterase Recognition

机译：矢量印迹杂交纳米膜的乙酰胆碱酯酶识别。

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摘要

Effective recognition of enzymatically active tetrameric acetylcholinesterase (AChE) is accomplished by a hybrid nanofilm composed of a propidium-terminated self-assembled monolayer (Prop-SAM) which binds AChE via its peripheral anionic site (PAS) and an ultrathin electrosynthesized molecularly imprinted polymer (MIP) cover layer of a novel carboxylate-modified derivative of 3,4-propylenedioxythiophene. The rebinding of the AChE to the MIP/Prop-SAM nanofilm covered electrode is detected by measuring in situ the enzymatic activity. The oxidative current of the released thiocholine is dependent on the AChE concentration from approximate to 0.04 x 10(-6) to 0.4 x 10(-6)m. An imprinting factor of 9.9 is obtained for the hybrid MIP, which is among the best values reported for protein imprinting. The dissociation constant characterizing the strength of the MIP-AChE binding is 4.2 x 10(-7)m indicating the dominant role of the PAS-Prop-SAM interaction, while the benefit of the MIP nanofilm covering the Prop-SAM layer is the effective suppression of the cross-reactivity toward competing proteins as compared with the Prop-SAM. The threefold selectivity gain provided by i) the shape-specific MIP filter, ii) the propidium-SAM, iii) signal generation only by the AChE bound to the nanofilm shows promise for assessing AChE activity levels in cerebrospinal fluid.
机译：有效的酶促四聚体乙酰胆碱酯酶（AChE）的识别是通过杂化纳米膜完成的，该杂化膜由一个端接自组装的丙啶的单层膜（Prop-SAM）和一个超薄的电合成分子印迹聚合物（Pros-SAM）通过其外围阴离子位点（PAS）与AChE结合（ MIP）的3,4-丙二氧基噻吩的新型羧酸酯修饰衍生物的覆盖层。通过原位测量酶活性来检测AChE与MIP / Prop-SAM纳米膜覆盖的电极的重新结合。释放的硫代胆碱的氧化电流取决于AChE浓度，范围从大约0.04 x 10（-6）到0.4 x 10（-6）m。杂交MIP获得的印迹因子为9.9，这是报道的蛋白质印迹的最佳值之一。表征MIP-AChE结合强度的解离常数为4.2 x 10（-7）m，表明PAS-Prop-SAM相互作用的主要作用，而覆盖Prop-SAM层的MIP纳米膜的好处是有效的与Prop-SAM相比，抑制了对竞争蛋白的交叉反应。 i）特定形状的MIP过滤器，ii）丙啶-SAM，iii）仅由与纳米膜结合的AChE产生的信号提供了三倍的选择性增益，显示了评估脑脊液中AChE活性水平的希望。

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