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Nano-Self-Assembly of Nucleic Acids Capable of Transfection without a Gene Carrier

机译：无需基因载体即可转染的核酸的纳米自组装体

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摘要

Nonviral gene carriers based on electrostatic interaction, encapsulation, or absorption require a large amount of polymer carrier to achieve reasonable transfection efficiencies. With cationic nanoparticles, for example, genes interact only with the surface of the nanoparticles, resulting in a low surface area to volume ratio (SA/V = 3/r). A large volume of carrier, therefore, is required to deliver a small copy number of genes. In this study, it is demonstrated that a nano-self-assembly of nucleic acids transfects itself into cells spontaneously, without the need for a gene carrier. The cellular uptake of this nanoassembly occurs through a number of endocytosis mechanisms. Once within the cell, the nanoassembly can escape endolysosomal vesicles and facilitate gene transfection. This nano-self-assembly consisting of zinc and plasmid DNA or siRNA, termed the Zn/DNA or Zn/siRNA nanocluster, is formed through the binding of Zn2+ ions to the phosphate groups of nucleic acids. The method described in this paper represents a new platform for carrier-free gene delivery that can be used to deliver any plasmid DNA or siRNA without the requirement for a specific modification in the nucleic acids or complicated steps to prepare dense particles.
机译：基于静电相互作用，封装或吸收的非病毒基因载体需要大量的聚合物载体才能实现合理的转染效率。例如，对于阳离子纳米粒子，基因仅与纳米粒子的表面相互作用，导致表面积与体积之比低（SA / V = 3 / r）。因此，需要大量的载体来递送少量的基因。在这项研究中，证明了核酸的纳米自组装可自动将其自身转染到细胞中，而无需基因载体。这种纳米组件的细胞摄取通过多种内吞作用机制发生。一旦进入细胞，纳米组件就可以逃逸溶酶体囊泡并促进基因转染。这种由锌和质粒DNA或siRNA组成的纳米自组装体，称为Zn / DNA或Zn / siRNA纳米簇，是通过Zn2 +离子与核酸磷酸基团的结合而形成的。本文描述的方法代表了一种新的无载体基因递送平台，可用于递送任何质粒DNA或siRNA，而无需对核酸进行特定修饰或制备致密颗粒的复杂步骤。

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  • 来源
    《Advanced Functional Materials》 |2015年第34期|5445-5451|共7页
  • 作者

    Lim Kwang Suk; Lee Daniel Y.; Valencia Gabriel M.; Won Young-Wook; Bull David A.;

  • 作者单位

    Univ Utah, Sch Med, Dept Surg, Div Cardiothorac Surg, Salt Lake City, UT 84132 USA;

    Univ Utah, Sch Med, Dept Surg, Div Cardiothorac Surg, Salt Lake City, UT 84132 USA|Univ Utah, Dept Pharmaceut & Pharmaceut Chem, Salt Lake City, UT 84112 USA;

    Univ Utah, Sch Med, Dept Surg, Div Cardiothorac Surg, Salt Lake City, UT 84132 USA;

    Univ Utah, Sch Med, Dept Surg, Div Cardiothorac Surg, Salt Lake City, UT 84132 USA|Univ Utah, Dept Pharmaceut & Pharmaceut Chem, Salt Lake City, UT 84112 USA;

    Univ Utah, Sch Med, Dept Surg, Div Cardiothorac Surg, Salt Lake City, UT 84132 USA|Univ Utah, Dept Pharmaceut & Pharmaceut Chem, Salt Lake City, UT 84112 USA;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    carrier-free gene delivery; divalent metal ions; gene delivery; zinc chloride;

    机译：无载体基因传递;二价金属离子;基因传递;氯化锌;

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