Nucleoside Triphosphates as Promoters to Enhance Nanoceria Enzyme-like Activity and for Single-Nucleotide Polymorphism Typing

Can Xu; Zhen Liu; Li Wu; Jinsong Ren; Xiaogang Qu

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Nucleoside Triphosphates as Promoters to Enhance Nanoceria Enzyme-like Activity and for Single-Nucleotide Polymorphism Typing

机译：核苷三磷酸作为促进剂，以增强纳米氧化铈酶样活性和单核苷酸多态性打字。

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Nucleoside triphosphates (NTPs) can improve the oxidase-like activity of nanoceria and the enhancement is correlated with the type of NTP. This effect is demonstrated to be as a result of the coupling of the oxidative reaction with the NTP hydrolysis reactions, as the nanoceria has both oxidase-like and phosphatase-like activities. The differences reflect the different dephosphorylation catalytic activities of nanoceria to the NTP used. Furthermore, based on the NTP-promoted oxidase-like activity of nanoceria and the differences among the different types of NTPs, series effective and high-throughput colorimetric assays for single-nucleotide polymorphism (SNP) typing are developed.

机译：核苷三磷酸酯（NTPs）可以改善纳米氧化铈的氧化酶样活性，并且这种增强与NTP类型有关。由于纳米氧化铈具有氧化酶样和磷酸酶样活性，因此证明该作用是氧化反应与NTP水解反应偶联的结果。差异反映了纳米氧化铈对所用NTP的不同去磷酸催化活性。此外，基于NTP促进的纳米氧化铈样活性和不同类型NTP之间的差异，开发了一系列有效和高通量比色法用于单核苷酸多态性（SNP）分型。

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来源
《Advanced Functional Materials》 |2014年第11期|1624-1630|共7页
作者
Can Xu; Zhen Liu; Li Wu; Jinsong Ren; Xiaogang Qu;
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Laboratory of Chemical Biology Division of Biological Inorganic Chemistry State Key laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Graduate School of the Chinese Academy of Sciences Chinese Academy of Sciences Changchun, Jilin, 130022, China;

Laboratory of Chemical Biology Division of Biological Inorganic Chemistry State Key laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Graduate School of the Chinese Academy of Sciences Chinese Academy of Sciences Changchun, Jilin, 130022, China;

Laboratory of Chemical Biology Division of Biological Inorganic Chemistry State Key laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Graduate School of the Chinese Academy of Sciences Chinese Academy of Sciences Changchun, Jilin, 130022, China;

Laboratory of Chemical Biology Division of Biological Inorganic Chemistry State Key laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Graduate School of the Chinese Academy of Sciences Chinese Academy of Sciences Changchun, Jilin, 130022, China;

Laboratory of Chemical Biology Division of Biological Inorganic Chemistry State Key laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Graduate School of the Chinese Academy of Sciences Chinese Academy of Sciences Changchun, Jilin, 130022, China;

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机译：猪乳糖酶基因启动子和增强子的单核苷酸多态性及活性分析
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6. The G/G Genotype of a Resistin Single-Nucleotide Polymorphism at −420 Increases Type 2 Diabetes Mellitus Susceptibility by Inducing Promoter Activity through Specific Binding of Sp1/3 [O] . Haruhiko Osawa, Kazuya Yamada, Hiroshi Onuma, 2004

机译：抵抗素单核苷酸多态性在-420的G / G基因型通过Sp1 / 3的特异性结合诱导启动子活性从而增加2型糖尿病的易感性。
7. The G/G Genotype of a Resistin Single-Nucleotide Polymorphism at −420 Increases Type 2 Diabetes Mellitus Susceptibility by Inducing Promoter Activity through Specific Binding of Sp1/3 [O] . Osawa, Haruhiko, Yamada, Kazuya, Onuma, Hiroshi, 2004

机译：抵抗素单核苷酸多态性在-420的G / G基因型通过Sp1 / 3的特异性结合诱导启动子活性，从而增加2型糖尿病的易感性。

Nucleoside Triphosphates as Promoters to Enhance Nanoceria Enzyme-like Activity and for Single-Nucleotide Polymorphism Typing

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