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Hierarchically Nanoporous Bioactive Classes for High Efficiency Immobilization of Enzymes

机译：用于高效固定酶的分层纳米多孔生物活性类别

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摘要

Bioactive glasses with hierarchical nanoporosity and structures have been heavily involved in immobilization of enzymes. Because of meticulous design and ingenious hierarchical nanostructuration of porosities from yeast cell biotemplates, hierarchically nanostructured porous bioactive glasses can provide a simple, cost-effective way to enhance catalytic activity of directly immobilized enzyme. Its unique chemical surface properties and hierarchical meso/macroporous structures lead to highly efficient catalytic performances of the directly immobilized enzymes. The enzyme molecules were spontaneously entrapped into the highly curved macropores (200-500 nm) via multipoint metal ion binding in electrical double layers. Hence, the enzyme activity and enzyme loading were enhanced, the cost of enzyme use was reduced, showing higher thermal and storage stabilities than free enzyme. The reactant and products of catalytic reactions can freely diffuse through open mesopores (2-40 nm). The formation mechanism of hierarchically structured porous bioactive glasses, the immobilization mechanism of enzyme and the catalysis mechanism of immobilized enzyme are then discussed. The novel nanostruc-ture with advanced properties is expected to be utilized as a solid support for any enzyme for bioconversion, bioremediation, biosensors and drugs.
机译：具有分级的纳米多孔性和结构的生物活性玻璃已经大量参与了酶的固定化。由于精心设计和来自酵母细胞生物模板的孔隙的巧妙的分级纳米结构，分级纳米结构的多孔生物活性玻璃可以提供一种简单，经济高效的方法来增强直接固定化酶的催化活性。其独特的化学表面性质和分级的介孔/大分子结构导致直接固定化酶的高效催化性能。通过双电层中的多点金属离子结合，酶分子自发地进入高度弯曲的大孔（200-500 nm）。因此，提高了酶活性和酶载量，降低了酶的使用成本，显示了比游离酶更高的热稳定性和储存稳定性。催化反应的反应物和产物可以通过开放的中孔（2-40 nm）自由扩散。然后讨论了分层结构的多孔生物活性玻璃的形成机理，酶的固定机理和固定化酶的催化机理。具有先进特性的新型纳米结构有望用作生物转化，生物修复，生物传感器和药物中任何酶的固体支持物。

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  • 来源
    《Advanced Functional Materials》 |2014年第15期|2206-2215|共10页
  • 作者

    Wen He; Dandan Min; Xudong Zhang; Yang Zhang; Zhiying Bi; Yuanzheng Yue;

  • 作者单位

    Shandong Key Laboratory of Class and Functional Ceramics Qilu University of Technology Jinan, 250353, PR China;

    Shandong Key Laboratory of Class and Functional Ceramics Qilu University of Technology Jinan, 250353, PR China;

    Shandong Key Laboratory of Class and Functional Ceramics Qilu University of Technology Jinan, 250353, PR China;

    College of Engineering Ocean University of China Qingdao, 266100, China;

    Shandong Key Laboratory of Class and Functional Ceramics Qilu University of Technology Jinan, 250353, PR China;

    Shandong Key Laboratory of Class and Functional Ceramics Qilu University of Technology Jinan, 250353, PR China,Section of Chemistry Aalborg University DK-9000 Aalborg, Denmark;

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