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首页> 外文期刊>Advanced Functional Materials >High Precision, Electrochemical Detection of Reversible Binding of Recombinant Proteins on Wide Bandgap GaN Electrodes Functionalized with Biomembrane Models
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High Precision, Electrochemical Detection of Reversible Binding of Recombinant Proteins on Wide Bandgap GaN Electrodes Functionalized with Biomembrane Models

机译：高精度，化学检测重组蛋白在生物膜模型功能化的宽带隙GaN电极上的可逆结合

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摘要

We report a novel hybrid charge sensor realized by the deposition of phos-pholipid monolayers on highly doped n-GaN electrodes. To detect the binding of recombinant proteins with histidine-tags, lipid vesicles containing chelator lipids were deposited on CaN electrodes pre-coated with octadecyltrimethox-ysilane monolayers. Owing to its optical transparency, CaN allows the confirmation of the fluidity of supported membranes by fluorescence recovery after photo-bleaching (FRAP). The electrolyte-(organic) insulator-semiconductor (EIS) setup enables one to transduce variations in the surface charge density ΔQ into a change in the interface capacitance ΔC_p and, thus, the flat-band potential ΔU_(FB). The obtained results demonstrate that the membrane-based charge sensor can reach a high sensitivity to detect reversible changes in the surface charge density on the membranes by the formation of chelator complexes, docking of eGFP with histidine tags, and cancellation by EDTA. The achievable resolution of ΔQ ≥ 0.1 μC/cm~2 is better than that obtained for membrane-functionalized p-GaAs, 0.9 μC/cm~2, and for ITO coated with a polymer supported lipid monolayer, 2.2 μC/cm~2. Moreover, we examined the potential application of optically active InGaN/GaN quantum dot structures, for the detection of changes in the surface potential from the photolumines-cence signals measured at room temperature.
机译：我们报告了通过在高掺杂n-GaN电极上沉积磷脂单分子层实现的新型混合电荷传感器。为了检测重组蛋白与组氨酸标签的结合，将含有螯合脂质的脂质囊泡沉积在预先涂有十八烷基三甲氧基-硅烷硅烷单层的CaN电极上。由于其光学透明性，CaN可以通过光漂白（FRAP）后的荧光回收来确认支撑膜的流动性。电解质（有机）绝缘体半导体（EIS）设置可以将表面电荷密度ΔQ的变化转换为界面电容ΔC_p的变化，从而将平带电势ΔU_（FB）转换。获得的结果表明，基于膜的电荷传感器可以通过形成螯合剂复合物，eGFP与组氨酸标签的对接以及EDTA的取消来检测膜表面电荷密度的可逆变化，从而达到很高的灵敏度。可获得的ΔQ≥0.1μC/ cm〜2的分辨率优于膜功能化的p-GaAs 0.9μC/ cm〜2和涂有聚合物支撑的脂质单层的ITO的分辨率为2.2μC/ cm〜2更好。此外，我们检查了光学活性InGaN / GaN量子点结构的潜在应用，以根据室温下测量的光致发光信号检测表面电势的变化。

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  • 来源
    《Advanced Functional Materials》 |2014年第31期|4927-4934|共8页
  • 作者

    Nataliya Frenkel; Jens Wallys; Sara Lippert; Joerg Teuhert; Stefan Kaufmann; Aparna Das; Eva Monroy; Martin Eickhoff; Motomu Tanaka;

  • 作者单位

    Physical Chemistry of Biosystems Institute of Physical Chemistry University of Heidelberg 69120, Heidelberg, Germany,Cell Biophysics Lab Institute of Toxicology and Genetics Karlsruhe Institute of Technology 76021, Karlsruhe, Germany;

    I Physikalisches Institut Justus-Liebig-Universitaet Giessen Heinrich-Buff-Ring 16 35392 Giessen, Germany;

    I Physikalisches Institut Justus-Liebig-Universitaet Giessen Heinrich-Buff-Ring 16 35392 Giessen, Germany;

    I Physikalisches Institut Justus-Liebig-Universitaet Giessen Heinrich-Buff-Ring 16 35392 Giessen, Germany;

    Physical Chemistry of Biosystems Institute of Physical Chemistry University of Heidelberg 69120, Heidelberg, Germany;

    CEA Grenoble, INAC/SP2M/NPSC 17 rue des Martyrs F-38054 Grenoble 9, France;

    CEA Grenoble, INAC/SP2M/NPSC 17 rue des Martyrs F-38054 Grenoble 9, France;

    I Physikalisches Institut Justus-Liebig-Universitaet Giessen Heinrich-Buff-Ring 16 35392 Giessen, Germany;

    Physical Chemistry of Biosystems Institute of Physical Chemistry University of Heidelberg 69120, Heidelberg, Germany,Cell Biophysics Lab Institute of Toxicology and Genetics Karlsruhe Institute of Technology 76021, Karlsruhe, Germany,Institute for Integrated Cell-Material Science (WPI iCeMS) Kyoto University 6068501, Kyoto, Japan;

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