• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Advanced Functional Materials >DNA-Templated Biomimetic Enzyme Sheets on Carbon Nanotubes to Sensitively In Situ Detect Superoxide Anions Released from Cells
【24h】

DNA-Templated Biomimetic Enzyme Sheets on Carbon Nanotubes to Sensitively In Situ Detect Superoxide Anions Released from Cells

机译：碳纳米管上的DNA模板仿生酶片可灵敏地检测从细胞释放的超氧阴离子

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Superoxide anion (O_2~- ) is implicated in a wide variety of biological phenomena and oxidative stress-related diseases. The electrochemical detection of O_2~- is very attractive but relies on superoxide dismutase enzymes, thus suffering from high cost and low durability. The advances of nanoscience allows architecting while functionalizing a biomimetic sensing platform in nanoscales for high sensitivity and specificity. In this work, manganous phosphate (Mn_3(PO_4)_2) nanosheets, a biomimetic enzyme, are template-synthesized with DNA and further assembled on carbon nanotubes (CNTs) to form unique DNA-Mn_3(PO_4)_2-CNT nanocomposite sheets, of which the Mn_3(PO_4)_2 sheets efficiently catalyze the dismutation of O_2~- while CNTs enable fast electron transfer, thus achieving highly sensitive and specific detection of O_2~-with long-term stability. The biomimetic O_2~- sensor is further used to monitor O_2~- in situ released from mouse cancer cell and normal skin cell under drug stimulation, showing excellent real time quantitative detection capability. This work demonstrates a nanoscale approach to not only synthesize but also design a biomimetic enzyme for comparable performance with the natural enzyme-based biosensor while rendering much higher durability than the natural one and thus holding a great promise for broad applications in fundamental research, clinic diagnostics and screening for drug therapy effects.
机译：超氧阴离子（O_2〜-）与多种生物学现象和氧化应激相关疾病有关。 O_2〜-的电化学检测非常吸引人，但依赖于超氧化物歧化酶，因此成本高，耐久性差。纳米科学的进步使建筑师能够在纳米级仿生传感平台功能化的同时实现高灵敏度和高特异性。在这项工作中，磷酸仿生酶磷酸锰（Mn_3（PO_4）_2）纳米片与模板进行模板合成，然后进一步组装在碳纳米管（CNT）上，形成独特的DNA-Mn_3（PO_4）_2-CNT纳米复合片。 Mn_3（PO_4）_2薄片能有效地催化O_2〜-的歧化，而CNTs能够实现快速的电子转移，从而实现了对O_2〜-的高灵敏度和特异性检测，并具有长期稳定性。仿生O_2〜-传感器还用于监测药物刺激下从小鼠癌细胞和正常皮肤细胞释放的O_2〜-，具有良好的实时定量检测能力。这项工作展示了一种纳米尺度的方法，不仅可以合成而且还可以设计一种仿生酶，使其具有与基于天然酶的生物传感器相当的性能，同时比天然酶具有更高的耐久性，因此在基础研究，临床诊断中具有广阔的应用前景并筛选药物治疗效果。

著录项

  • 来源
    《Advanced Functional Materials》 |2014年第37期|5897-5903|共7页
  • 作者

    Xiaoqing Ma; Weihua Hu; Chunxian Guo; Ling Yu; Lixia Gao; Jiale Xie; Chang Ming Li;

  • 作者单位

    Institute for Clean Energy & Advanced Materials Southwest University Chongqing 400715, PR China,Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies Chongqing 400715, PR China,Faculty of Materials and Energy Southwest University Chongqing 400715, PR China;

    Institute for Clean Energy & Advanced Materials Southwest University Chongqing 400715, PR China,Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies Chongqing 400715, PR China,Faculty of Materials and Energy Southwest University Chongqing 400715, PR China;

    Institute for Clean Energy & Advanced Materials Southwest University Chongqing 400715, PR China,Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies Chongqing 400715, PR China,Faculty of Materials and Energy Southwest University Chongqing 400715, PR China;

    Institute for Clean Energy & Advanced Materials Southwest University Chongqing 400715, PR China,Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies Chongqing 400715, PR China,Faculty of Materials and Energy Southwest University Chongqing 400715, PR China;

    Institute for Clean Energy & Advanced Materials Southwest University Chongqing 400715, PR China,Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies Chongqing 400715, PR China,Faculty of Materials and Energy Southwest University Chongqing 400715, PR China;

    Institute for Clean Energy & Advanced Materials Southwest University Chongqing 400715, PR China,Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies Chongqing 400715, PR China,Faculty of Materials and Energy Southwest University Chongqing 400715, PR China;

    Institute for Clean Energy & Advanced Materials Southwest University Chongqing 400715, PR China,Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energies Chongqing 400715, PR China,Faculty of Materials and Energy Southwest University Chongqing 400715, PR China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号