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首页> 外文期刊>Advanced Functional Materials >Fabrication of Nanofiber Microarchitectures Localized within Hydrogel Microparticles and Their Application to Protein Delivery and Cell Encapsulation
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Fabrication of Nanofiber Microarchitectures Localized within Hydrogel Microparticles and Their Application to Protein Delivery and Cell Encapsulation

机译：水凝胶微粒内的纳米纤维微体系结构的制备及其在蛋白质传递和细胞封装中的应用

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摘要

A simple method to generate well-defined microscopic architectures composed of electrospun nanofibers is reported and their potential application to biomedical fields are described. The photopatterning of polyethylene glycol (PEC) hydrogel on electrospun polycarprolactone (PCL) nanofibers leads to the formation of two different microdomains in nanofibrous mats: a bare nanofiber region and a hydrogel-entrapped nanofiber region. The selective dissolution of bare nanofibers with an organic solvent that cannot penetrate the PEC hydrogel enables the localization of PCL nanofibers within the hydrogel microstructures, thus generating microarchitectured nanofibers. The resultant microarchitectures are easily detached from the substrate by the water-induced swelling of the PEC hydrogel. Microparticles are ultimately obtained, the size and shape of which can be easily controlled with proper photomask designs. In proof of concept experiments, bovine serum albumin(BSA)-loaded PCL nanofibers that are entrapped within the hydrogel microparticles are prepared and the sustained release of BSA from micro-patterned nanofibers is successfully demonstrated, indicating the potential application of the proposed microarchitectured nanofibers to drug delivery systems. For another possible application, the capability of the nanofiber-incorporated hydrogel to encapsulate mammalian cells is investigated and the incorporation of nanofibers within the PEC hydrogel promoted cell adhesion and spreading when compared with bare PEC hydrogel is confirmed.
机译：报告了一种简单的方法来生成由电纺纳米纤维组成的定义明确的微观结构，并描述了它们在生物医学领域中的潜在应用。聚乙二醇（PEC）水凝胶在静电纺丝聚己内酯（PCL）纳米纤维上的光图案化导致在纳米纤维垫中形成两个不同的微区：裸露的纳米纤维区域和包裹水凝胶的纳米纤维区域。用不能穿透PEC水凝胶的有机溶剂选择性溶解裸露的纳米纤维，可以使PCL纳米纤维在水凝胶微结构内定位，从而生成微结构化的纳米纤维。通过水诱导的PEC水凝胶溶胀，很容易将所得的微体系结构从基材上剥离下来。最终获得了微粒，可以通过适当的光掩模设计轻松控制微粒的大小和形状。在概念验证实验中，制备了包埋在水凝胶微粒中的牛血清白蛋白（BSA）负载PCL纳米纤维，并成功证明了BSA从微图案纳米纤维中的持续释放，表明拟议的微体系结构纳米纤维在纳米纤维上的潜在应用药物输送系统。对于另一种可能的应用，研究了掺有纳米纤维的水凝胶包封哺乳动物细胞的能力，并且证实了与裸PEC水凝胶相比，在PEC水凝胶中掺入纳米纤维可促进细胞粘附和扩散。

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  • 来源
    《Advanced Functional Materials》 |2013年第5期|591-597|共7页
  • 作者

    Hyun jong Lee; Young Ha Park; Won-Gun Koh;

  • 作者单位

    Active Polymer Center for Pattern Integration Department of Chemical and Biomolecular Engineering Yonsei University 50 Yonsei-ro, Seodaemun-gu Seoul 120-749, South Korea;

    Active Polymer Center for Pattern Integration Department of Chemical and Biomolecular Engineering Yonsei University 50 Yonsei-ro, Seodaemun-gu Seoul 120-749, South Korea;

    Active Polymer Center for Pattern Integration Department of Chemical and Biomolecular Engineering Yonsei University 50 Yonsei-ro, Seodaemun-gu Seoul 120-749, South Korea;

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