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首页> 外文期刊>Advanced Functional Materials >Dual-Functional Poly(3,4-ethylenedioxythiophene)/MnO_2 Nanoellipsoids for Enhancement of Neurite Outgrowth and Exocytosed Biomolecule Sensing in PCI2 Cells
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Dual-Functional Poly(3,4-ethylenedioxythiophene)/MnO_2 Nanoellipsoids for Enhancement of Neurite Outgrowth and Exocytosed Biomolecule Sensing in PCI2 Cells

机译：双功能聚（3,4-乙撑二氧噻吩）/ MnO_2纳米椭球体可增强PCI2细胞的神经突生长和胞外生物分子传感。

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摘要

Dual-functional MnO_2-decorated poly(3,4-ethylenedioxythiophene) (PEDOT/ MnO_2) nanoellipsoids are fabricated for enhancement of neurite outgrowth during differentiation and real-time monitoring of PCI 2 cells. The PEDOT nanoellipsoids are prepared by chemical oxidation polymerization in reverse microemulsion and the MnO_2 domains on the surface of the PEDOT are formed by redox deposition. A PCI 2 cell is used as a model cell for neuronal differentiation. The morphology, differentiation efficiency, average neurite length, expression level of DMT1, phosphorylation of ERK1/2, and viability of PCI 2 cells upon the exposure of the PEDOT/MnO_2 nanoellipsoids are examined. The PEDOT/MnO_2 nanoellipsoids facilitate neurite outgrowth in proportion to the dose of the nanoellipsoids, and MnO_2 domains play a pivotal role in facilitation effects on cell differentiation. The PEDOT/MnO_2 nanoellipsoids represent low toxicity in the cells due to biocompatible PEDOT matrix. Moreover, the PEDOT/MnO_2 nanoellipsoids are further applied as a transducer material for label-free real-time monitoring of PCI 2 cells. The exocytosed catecholamines from living cells are successfully detected. The dual-function-alized PEDOT/MnO_2 nanoellipsoids may be used in tissue engineering related to the development and monitoring of mammalian nervous systems.
机译：制备了双功能MnO_2修饰的聚（3,4-乙撑二氧噻吩）（PEDOT / MnO_2）纳米椭球体，用于增强PCI 2细胞分化和实时监测过程中的神经突生长。通过在逆向微乳液中进行化学氧化聚合反应制备PEDOT纳米椭圆体，并通过氧化还原沉积在PEDOT表面形成MnO_2域。 PCI 2细胞用作神经元分化的模型细胞。检测了PEDOT / MnO_2纳米椭圆体暴露后的形态，分化效率，平均神经突长度，DMT1的表达水平，ERK1 / 2的磷酸化以及PCI 2细胞的活力。 PEDOT / MnO_2纳米椭球体与纳米椭球体的剂量成比例促进神经突向外生长，而MnO_2域在促进细胞分化方面起着关键作用。由于生物相容性PEDOT基质，PEDOT / MnO_2纳米椭球体在细胞中表现出低毒性。此外，PEDOT / MnO_2纳米椭球体还被用作换能器材料，用于PCI 2细胞无标签实时监测。成功地检测到了活细胞中胞吐出的儿茶酚胺。双重功能化的PEDOT / MnO_2纳米椭圆体可用于与哺乳动物神经系统发育和监测有关的组织工程。

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  • 来源
    《Advanced Functional Materials》 |2013年第15期|1947-1956|共10页
  • 作者

    Sojin Kim; Wcm-Kyu Oh; Yoon Seonjeong; Jyongsik Jang;

  • 作者单位

    World Class University (WCU) program of Chemical Convergence for Energy &. Environment (C2E2) ,School of Chemical and Biological Engineering Seoul National University;

    World Class University (WCU) program of Chemical Convergence for Energy &. Environment (C2E2) ,School of Chemical and Biological Engineering Seoul National University;

    World Class University (WCU) program of Chemical Convergence for Energy &. Environment (C2E2) ,School of Chemical and Biological Engineering Seoul National University;

    World Class University (WCU) program of Chemical Convergence for Energy &. Environment (C2E2) ,School of Chemical and Biological Engineering Seoul National University;

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