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Synthesis, Characterization, and Application of Antibody Functionalized Fluorescent Silica Nanoparticles

机译:抗体功能化荧光二氧化硅纳米粒子的合成,表征与应用

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摘要

Fluorescent silica nanoparticles (FSNs) are prepared by incorporating dye into a mesoporous silica nanoparticle (MSN) synthesis procedure. FSNs containing sulforhodamine B, hydrophobically modified sulforhodamine B, and Cascade Blue hydrazide are made. The MSN-based FSNs do not leach dye under simulated physiological conditions and have strong, stable fluorescence. FSNs prepared with sulforhodamine B are compared to FSNs prepared with hydrophobically modified sulforhodamine B. The data indicate that FSNs prepared with sulforhodamine B are equally as stable but twice as fluorescent as particles made with hydrophobically modified sulforhodamine B. The fluorescence of a FSN prepared with sulforhodamine B is 10 times more intense than the fluorescence of a 4.5 nm core-shell CdSe/ZnS quantum dot. For diagnostic applications, a method to selectively and covalently bind antibodies to the surface of the FSNs is devised. FSNs that are functionalized with antibodies specific for Neisseria gonorrhoeae specifically bind to Neis-seria gonorrhoeae in flow cytometry experiments, thus demonstrating the functionality of the attached antibodies and the potential of MSN-based FSNs to be used in diagnostic applications.
机译:通过将染料掺入介孔二氧化硅纳米粒子(MSN)的合成程序中,可以制备荧光二氧化硅纳米粒子(FSN)。制备了含有磺基若丹明B,疏水改性的磺基若丹明B和级联蓝酰肼的FSN。基于MSN的FSN在模拟的生理条件下不会浸出染料,并具有强而稳定的荧光。将用磺基若丹明B制备的FSN与用疏水改性的磺基若丹明B制备的FSN进行了比较。数据表明,用磺基若丹明B制备的FSN具有与疏水性改性的磺基若丹明B制备的颗粒同等的稳定性,但其荧光是其两倍。 B的强度是4.5 nm核壳CdSe / ZnS量子点的荧光强度的10倍。对于诊断应用,设计了一种将抗体选择性和共价结合至FSN表面的方法。在流式细胞术实验中,用淋病奈瑟氏球菌特异性抗体功能化的FSN与淋病奈瑟氏球菌特异性结合,因此证明了所附着抗体的功能性以及基于MSN的FSN在诊断应用中的潜力。

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  • 来源
    《Advanced Functional Materials》 |2013年第26期|3335-3343|共9页
  • 作者单位

    Department of Chemistry and Biochemistry University of Maryland College Park, College Park, MD 20742, USA;

    Department of Chemistry Wichita State University Wichita, KS 67260, USA;

    Department of Chemistry and Biochemistry University of Maryland College Park, College Park, MD 20742, USA;

    Department of Chemistry and Biochemistry University of Maryland College Park, College Park, MD 20742, USA;

    Department of Chemistry and Biochemistry University of Maryland College Park, College Park, MD 20742, USA;

    Department of Chemistry Wichita State University Wichita, KS 67260, USA;

    Department of Chemistry and Biochemistry University of Maryland College Park, College Park, MD 20742, USA;

    Department of Cell Biology and Molecular Genetics University of Maryland College Park, College Park, MD 20742, USA;

    Department of Chemistry and Biochemistry University of Maryland College Park, College Park, MD 20742, USA;

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