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首页> 外文期刊>Advanced Functional Materials >Engineered 3D Silk-Based Metastasis Models: Interactions Between Human Breast Adenocarcinoma, Mesenchymal Stem Cells and Osteoblast-Like Cells
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Engineered 3D Silk-Based Metastasis Models: Interactions Between Human Breast Adenocarcinoma, Mesenchymal Stem Cells and Osteoblast-Like Cells

机译：工程化的基于丝绸的3D转移模型：人乳腺癌，间充质干细胞和成骨细胞样细胞之间的相互作用

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摘要

Bone metastasis occurs in 70％ of breast cancer patients and is a frequent cause of morbidity in cancer patients. A delicate balance exists in the bone microenvironment, but the functional dynamics underlying the tumor cell-microenvironment interactions remain poorly understood. 3D in vitro model systems of metastasis can throw new light on this phenomenon. Silk protein fibroin scaffolds, are cytocompatible for 3D cancer cell culture. They are structurally more resistant to protease degradation than other native biomaterials making these matrices suitable for cancer modeling. In this report, human breast adenocarcinoma cells, human osteoblast like cells and mesenchymal stem cells are co-cultered. Cancer cells and osteoblast-like cells are found to interact through secreted products. Decreased population of osteoblast-like cells and mineralization of extracellular matrix are observed as a result of co-culture. Significantly increased migration of breast cancer cells is observed in the bone-like constructs than in non-seeded scaffolds. The co-culture constructs show significant increase in drug resistance, invasiveness and angiogenicity. Co-culture of breast cancer cells with osteoblast like cells and mesenchymal stem cells also indicate that the interaction of cancer cells with bone microenvironment varies with spatial organization, presence of osteogenic factors as well as stromal cell type. Here, results show that 3D in vitro co-culture models is possibly a better system to study and target cancer progression.
机译：骨转移发生在70％的乳腺癌患者中，并且是癌症患者发病的常见原因。骨微环境之间存在微妙的平衡，但对肿瘤细胞与微环境相互作用的潜在功能动力学知之甚少。 3D体外转移模型系统可以为这一现象提供新的思路。丝蛋白纤维蛋白支架对于3D癌细胞培养具有细胞相容性。与其他天然生物材料相比，它们在结构上更耐蛋白酶降解，从而使这些基质适合癌症建模。在该报告中，人类乳腺腺癌细胞，人类成骨细胞样细胞和间充质干细胞被共同培养。发现癌细胞和成骨细胞样细胞通过分泌产物相互作用。共培养的结果是，成骨细胞样细胞数量减少，细胞外基质矿化。与非种子支架相比，在骨样结构中观察到乳腺癌细胞的迁移显着增加。共培养构建体显示出耐药性，侵袭性和血管生成性的显着提高。乳腺癌细胞与成骨细胞样细胞和间充质干细胞的共培养也表明癌细胞与骨微环境的相互作用随空间组织，成骨因子的存在以及基质细胞类型的不同而变化。在这里，结果表明3D体外共培养模型可能是研究和靶向癌症进展的更好系统。

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  • 来源
    《Advanced Functional Materials》 |2013年第42期|5249-5260|共12页
  • 作者

    SarmisthaTalukdar; SubhasC.Kundu;

  • 作者单位

    Department of Biotechnology Indian Institute ofTechnology Kharagpur 7213 02, India;

    Department of Biotechnology Indian Institute ofTechnology Kharagpur 7213 02, India;

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