• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Advanced Functional Materials >Spatially Controlled Delivery of siRNAs to Stem Cells in Implants Generated by Multi-Component Additive Manufacturing
【24h】

Spatially Controlled Delivery of siRNAs to Stem Cells in Implants Generated by Multi-Component Additive Manufacturing

机译：siRNA在多成分增材制造产生的植入物中向干细胞的空间控制递送。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Additive manufacturing is a promising technique in tissue engineering, as it enables truly individualized implants to be made to fit a particular defect. As previously shown, a feasible strategy to produce complex multicellular tissues is to deposit different small interfering RNA (siRNA) in porous implants that are subsequently sutured together. In this study, an additive manufacturing strategy to deposit carbohydrate hydrogels containing different siRNAs is applied into an implant, in a spatially controlled manner. When the obtained structures are seeded with mesenchymal stem (stromal) cells, the selected siRNAs are delivered to the cells and induces specific and localized gene silencing. Here, it is demonstrated how to replicate part of a patient's spinal cord from a computed tomography scan, using an additive manufacturing technique to produce an implant with compartmentalized siRNAs in the locations corresponding to distinct tissue. Hydrogel solutions loaded with different siRNA can be co-printed together with polycaprolactone that acts as rigid mechanical support to the hydrogel. This study demonstrates a new route for the production of 30 functionalized, individualized implants which may provide great clinical benefit.
机译：增材制造在组织工程中是一项很有前途的技术，因为它可以制造出真正个性化的植入物以适应特定的缺陷。如前所示，生产复杂的多细胞组织的可行策略是在随后植入在一起的多孔植入物中沉积不同的小干扰RNA（siRNA）。在这项研究中，以空间可控的方式将一种添加制造策略以沉积包含不同siRNA的碳水化合物水凝胶应用于植入物中。当将获得的结构植入间充质干（基质）细胞后，选定的siRNA会传递到细胞中并诱导特异性和局部基因沉默。在此，演示了如何使用增材制造技术从计算机断层扫描中复制患者脊髓的一部分，以生产在对应于不同组织的位置带有间隔化siRNA的植入物。载有不同siRNA的水凝胶溶液可与聚己内酯一起共印，聚己内酯可作为对水凝胶的刚性机械支持。这项研究表明了生产30种功能化，个性化植入物的新途径，这可能会提供巨大的临床益处。

著录项

  • 来源
    《Advanced Functional Materials》 |2013年第45期|5599-5607|共9页
  • 作者

    Morten Ostergaard Andersen; Dang Quang Svend Le; Muwan Chen; Jens Vinge Nygaard; Moustapha Kassem; Cody Buenger; Jorgen Kjems;

  • 作者单位

    Department of Molecular Biology and Genetics Aarhus University C. F. Moellers Alle 3, Building 1130,DK-8000 Aarhus C, Denmark,Interdisciplinary Nanoscience Center (iNANO), Aarhus University Gustav Wieds Vej 14, DK-8000 Aarhus C, Denmark,Orthopaedic Research Laboratory, Aarhus University Hospital N0rrebrogade44(la), DK-8000 Aarhus C, Denmark;

    Interdisciplinary Nanoscience Center (iNANO), Aarhus University Gustav Wieds Vej 14, DK-8000 Aarhus C, Denmark,Orthopaedic Research Laboratory, Aarhus University Hospital N0rrebrogade44(la), DK-8000 Aarhus C, Denmark;

    Interdisciplinary Nanoscience Center (iNANO), Aarhus University Gustav Wieds Vej 14, DK-8000 Aarhus C, Denmark,Orthopaedic Research Laboratory, Aarhus University Hospital N0rrebrogade44(la), DK-8000 Aarhus C, Denmark;

    Interdisciplinary Nanoscience Center (iNANO), Aarhus University Gustav Wieds Vej 14, DK-8000 Aarhus C, Denmark,Department of Engineering, Aarhus University,Finlandsgade22, DK-8000 Aarhus C, Denmark;

    Molecular Endocrinology Laboratory Odense University Hospital J. B. Winslaws Vej 25, DK-5000 Odense C, Denmark;

    Orthopaedic Research Laboratory, Aarhus University Hospital N0rrebrogade44(la), DK-8000 Aarhus C, Denmark;

    Department of Molecular Biology and Genetics Aarhus University C. F. Moellers Alle 3, Building 1130,DK-8000 Aarhus C, Denmark,Interdisciplinary Nanoscience Center (iNANO), Aarhus University Gustav Wieds Vej 14, DK-8000 Aarhus C, Denmark;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号