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首页> 外文期刊>Advanced Functional Materials >Highly Fluorescent Nanodiamonds Protein-Functionalized for Cell Labeling and Targeting
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Highly Fluorescent Nanodiamonds Protein-Functionalized for Cell Labeling and Targeting

机译：蛋白质功能强大的高度荧光纳米金刚石，可用于细胞标记和靶向

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摘要

Fluorescent nanodiamond (FND) is attracting much attention as a bioim-aging agent because of its inherent biocompatibility and superior optical properties (e.g., excellent photostability and far-red emission). However, for practical use in life science research, some issues such as higher brightness and ease of bioconjugation have to be solved. Here, it is shown that the 100-nm FND particles fabricated by using nitrogen-rich type Ib diamonds and high-energy proton irradiation are highly fluorescent and readily functional-izable with proteins for biological applications. In the first approach, acid-treated FND is noncovalently coated with glycoproteins or neoglycoproteins (i.e., proteins chemically modified with multiple sugar residues) for targeting hepatocytes via carbohydrate receptors. In the second approach, FND is first PEGylated and then covalently conjugated with streptavidin, to which biotin-labeled antibodies of interest are linked. High targeting specificity of the bioconjugated FND is demonstrated with the human hepatoma cell line, HepG2, and breast cancer cell lines, ASB145-1R, MCF-7, and MDA-MB-231 cells. These approaches should be widely applicable to a variety of situations for specific targeting and labeling of cells.
机译：荧光纳米金刚石（FND）由于其固有的生物相容性和优异的光学性能（例如，优异的光稳定性和远红光发射）而作为生物显像剂备受关注。然而，为了在生命科学研究中的实际应用，必须解决一些问题，例如更高的亮度和生物缀合的容易性。此处显示，通过使用富氮Ib金刚石和高能质子辐照制造的100 nm FND粒子具有很高的荧光性，并易于使用蛋白质进行生物学应用。在第一种方法中，酸处理的FND用糖蛋白或新糖蛋白（即用多个糖残基化学修饰的蛋白）非共价包覆，以通过碳水化合物受体靶向肝细胞。在第二种方法中，先将FND聚乙二醇化，然后与抗生蛋白链菌素共价缀合，然后将生物素标记的目标抗体连接到抗生蛋白链菌素上。用人类肝癌细胞系，HepG2和乳腺癌细胞系，ASB145-1R，MCF-7和MDA-MB-231细胞证明了生物缀合FND的高靶向特异性。这些方法应广泛适用于多种情况，以进行细胞的特定靶向和标记。

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  • 来源
    《Advanced Functional Materials》 |2013年第46期|5737-5745|共9页
  • 作者

    Be-Ming Chang; Hsin-Hung Lin; Long-Jyun Su; Wen-Der Lin; Ruey-Jen Lin; Yan-Kai Tzeng; Reiko T. Lee; Yuan C. Lee; Alice L.Yu; Huan-Cheng Chang;

  • 作者单位

    Institute of Atomic and Molecular Sciences Academia Sinica, Taipei 106, Taiwan,Taiwan International Graduate Program-Molecular Science and Technology Academia Sinica, Taipei 115 Taiwan,Department of Chemistry National Tsing Hua University Hsinchu 300, Taiwan;

    Genomics Research Center Academia Sinica, Taipei 115, Taiwan;

    Institute of Atomic and Molecular Sciences Academia Sinica, Taipei 106, Taiwan;

    Genomics Research Center Academia Sinica, Taipei 115, Taiwan;

    Genomics Research Center Academia Sinica, Taipei 115, Taiwan;

    Institute of Atomic and Molecular Sciences Academia Sinica, Taipei 106, Taiwan;

    Genomics Research Center Academia Sinica, Taipei 115, Taiwan;

    Genomics Research Center Academia Sinica, Taipei 115, Taiwan;

    Genomics Research Center Academia Sinica, Taipei 115, Taiwan;

    Institute of Atomic and Molecular Sciences Academia Sinica, Taipei 106, Taiwan,Taiwan International Graduate Program-Molecular Science and Technology Academia Sinica, Taipei 115 Taiwan;

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