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首页> 外文期刊>Advanced Functional Materials >Transfer Printing of Cell Layers with an Anisotropic Extracellular Matrix Assembly using Cell-Interactive and Thermosensitive Hydrogels
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Transfer Printing of Cell Layers with an Anisotropic Extracellular Matrix Assembly using Cell-Interactive and Thermosensitive Hydrogels

机译：使用细胞相互作用和热敏水凝胶通过各向异性的细胞外基质组装转移细胞层

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摘要

The structure of tissue plays a critical role in its function and therefore a great deal of attention has been focused on engineering native tissue-like constructs for tissue engineering applications. Transfer printing of cell layers is a new technology that allows controlled transfer of cell layers cultured on smart substrates with defined shape and size onto tissue-specific defect sites. Here, the temperature-responsive swelling-deswelling of the hydrogels with groove patterns and their versatile and simple use as a template to harvest cell layers with anisotropic extracellular matrix assembly is reported. The hydrogels with a cell-interactive peptide and anisotropic groove patterns are obtained via enzymatic polymerization. The results show that the cell layer with patterns can be easily transferred to new substrates by lowering the temperature. In addition, multiple cell layers are stacked on the new substrate in a hierarchical manner and the cell layer is easily transplanted onto a subcutaneous region. These results indicate that the evaluated hydrogel can be used as a novel substrate for transfer printing of artificial tissue constructs with controlled structural integrity, which may hold potential to engineer tissue that can closely mimic native tissue architecture.
机译：组织的结构在其功能中起着至关重要的作用，因此，很多注意力都集中在对组织工程应用中的天然组织样结构进行工程设计上。细胞层的转移印刷是一项新技术，可以将在智能基板上培养的具有确定形状和大小的细胞层控制转移到组织特异性缺损部位。在此，报道了具有沟槽图案的水凝胶的温度响应性溶胀-消溶及其作为模板以具有各向异性细胞外基质组装的细胞层的通用性和简单性。通过酶促聚合获得具有细胞相互作用肽和各向异性凹槽图案的水凝胶。结果表明，通过降低温度，具有图案的单元层可以容易地转移到新的基板上。另外，多个细胞层以分级的方式堆叠在新基底上，并且该细胞层易于移植到皮下区域上。这些结果表明，所评价的水凝胶可以用作具有受控结构完整性的人工组织构造的转移印花的新型底物，这可能具有工程化组织的潜力，该组织可以紧密地模仿天然组织的结构。

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  • 来源
    《Advanced Functional Materials》 |2012年第19期|4060-4069|共10页
  • 作者

    Indong Jun; Seok Joo Kim; Ji-Hye Lee; Young Jun Lee; Young Min Shin; Eunpyo Choi; Kyung Min Park; Jungyul Park; Ki Dong Park; Heungsoo Shin;

  • 作者单位

    Department of Bioengineering Hanyang University 17 Haengdang-dong, Seongdong-gu, Seoul133-791, Republic of Korea;

    Department of Bioengineering Hanyang University 17 Haengdang-dong, Seongdong-gu, Seoul133-791, Republic of Korea;

    Department of Bioengineering Hanyang University 17 Haengdang-dong, Seongdong-gu, Seoul133-791, Republic of Korea;

    Department of Bioengineering Hanyang University 17 Haengdang-dong, Seongdong-gu, Seoul133-791, Republic of Korea;

    Department of Bioengineering Hanyang University 17 Haengdang-dong, Seongdong-gu, Seoul133-791, Republic of Korea;

    Department of Mechanical Engineering Sogang University Sinsu-dong, Mapo-gu, Seoul 121-742, Republic of Korea;

    Department of Molecular Science and Technology Ajou University 5 WonChun-dong, Yeoungtong-gu, Suwon 443-749, Republic of Korea;

    Department of Mechanical Engineering Sogang University Sinsu-dong, Mapo-gu, Seoul 121-742, Republic of Korea;

    Department of Molecular Science and Technology Ajou University 5 WonChun-dong, Yeoungtong-gu, Suwon 443-749, Republic of Korea;

    Department of Bioengineering Hanyang University 17 Haengdang-dong, Seongdong-gu, Seoul133-791, Republic of Korea;

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