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Peptides for the Biofunctionalization of Silicon for Use in Optical Sensing with Porous Silicon Microcavities

机译：硅生物功能化的肽，用于多孔硅微腔的光学传感

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摘要

Addressing the surface chemistry of silicon is of fundamental scientific and technical significance due to the wide use of this material in electronics and optics. A novel method of functionalizing silicon (Si) via short peptides with binding specificity for Si is presented. The peptide presenting the highest affinity for Si is identified via phage display technology, and the 12-mer LLADTTHHRPWT and SPCLSLVSHMQT peptides were found to be specific for the n~+-Si and p~+-Si surfaces, respectively. In our sensing application, the obtained peptides are used as functionalizing linkers to allow porous silicon microcavities to bind biotin and then capture streptavidin. Molecular detection is monitored via reflectometric interference spectra as shifts in the resonance peaks of the cavity structure. An improved streptavidin sensing (21 times lower detection limit) with peptide-functionalized porous silicon microcavities is demonstrated, compared to sensing performed with devices functionalized with the commonly used silanization method, suggesting that the modification of Si via Si-specific peptides provides better interface layers for molecular detection. High-resolution atomic force microscopy images corroborate this result and reveal the formation of ordered nanometer-sized molecular layers when peptide-route functionalization is performed.
机译：由于硅材料在电子和光学领域的广泛应用，解决硅的表面化学具有根本的科学和技术意义。提出了一种通过对Si具有结合特异性的短肽官能化硅（Si）的新方法。通过噬菌体展示技术鉴定了对Si具有最高亲和力的肽，发现12聚体LLADTTHHRPWT和SPCLSLVSHMQT肽分别对n〜+ -Si和p〜+ -Si表面具有特异性。在我们的传感应用中，获得的肽用作功能化的连接子，以允许多孔硅微腔结合生物素，然后捕获链霉亲和素。分子检测通过反射干涉光谱作为腔结构共振峰的偏移进行监控。与使用常用硅烷化方法功能化的设备进行的传感相比，采用肽功能化的多孔硅微腔的链霉亲和素传感具有改进的检测灵敏度（检测限低21倍），表明通过Si特异性肽对Si进行修饰可提供更好的界面层用于分子检测。高分辨率原子力显微镜图像证实了这一结果，并揭示了在进行肽路线功能化时形成的有序纳米尺寸分子层。

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  • 来源
    《Advanced Functional Materials》 |2011年第11期|p.2003-2011|共9页
  • 作者

    Ellas Estephan; Ma He-Belle Saab; Vivechana Agarwal; Frédéric J. C. Cuisinier; Christian Larroque; Csilla Gergeiy;

  • 作者单位

    EA4203, UFROdontologie Universite Montpellier 1 34193 Montpellier Cedex 5, France Laboratoire Charles Coulomb UMR 5221 CNRS-Universite Montpellier 2 34095, Montpellier Cedex 5, France;

    Laboratoire Charles Coulomb UMR 5221, CNRS-Universite Montpellier 1 34095, Montpellier Cedex 5, France;

    CIICAP- Universidad Autonoma del Estado de Morelos Av. Universidad 1001 Col Chamilpa, Cuernavaca, Mor., Mexico;

    EA 4203, UFROdontologie Universite Montpellier 1 34193 Montpellier Cedex 5, France;

    IRCM/INSERM896 Centre Regional de Lutte contre le Cancer Val d'Aurelle - Paul Lamarque,Universite Montpellier 1 34298 Montpellier, France;

    Laboratoire Charles CoulombUMR 5221 CNRS-Universite Montpellier 234095, Montpellier Cedex 5, France;

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