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首页> 外文期刊>Advanced Functional Materials >Solid Free-Form Fabrication of Tissue-Engineering Scaffolds with a Poly(lactic-co-glycolic acid) Grafted Hyaluronic Acid Conjugate Encapsulating and Intact Bone Morphogenetic Protein-2/Poly(ethylene glycol) Complex
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Solid Free-Form Fabrication of Tissue-Engineering Scaffolds with a Poly(lactic-co-glycolic acid) Grafted Hyaluronic Acid Conjugate Encapsulating and Intact Bone Morphogenetic Protein-2/Poly(ethylene glycol) Complex

机译：固体自由形式的组织工程支架与聚乳酸-乙醇酸接枝的透明质酸共轭封装和完整的骨形态发生蛋白2 /聚（乙二醇）复合物。

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摘要

Despite wide applications of bone morprjogenetic protein-2 (BMP-2), there are few methods to incorporate BPM-2 within polymeric scaffolds while maintaining biological activity. Solid free-form fabrication (SFF) of tissue-engineering scaffold is successfully carried out with poly(lactic-co-glycolic acid) grafted hyaluronic acid (HA-PLGA) encapsulating intact BMP-2/ poly(ethylene glycol) (PEC) complex. HA-PLGA conjugate is synthesized in dimethyl sulfoxide (DMSO) by the conjugation reaction of adipic acid dihydrazide modified HA (HA-ADH) and PLGA activated with NX-dicyclohexylcarbodiimide (DCC) and N-hydroxysuccinimide (NHS). BMP-2 is comptexed with PEG, which is encapsulated within the PLGA domain of the HA-PLGA conjugate by SFF to prepare tissue-engineering scaffolds. In vitro release tests confirm the sustained release of intact BMP-2 from the scaffolds for up to a month. After confirmation of the enhanced osteoblast cell growth, and high gene-expression levels of alkaline phosphatase (ALP), osteocalcin (OC), and osterix (OSX) !n the cells, the HA-PLGA/PEG/BMP-2 scaffolds are implanted into calvarial bone defects of Sprague Dawley (SD) rats. Microcomputed tomography (jiCT) and histological analyses with Masson's trichrome, and hematoxylin and eosin (H&E) staining reveal effective bone regeneration on the scaffolds of HA-PLGA/PEG/BMP-2 blends.
机译：尽管骨致骨增生蛋白2（BMP-2）的广泛应用，很少有方法可以将BPM-2掺入聚合物支架中，同时保持其生物学活性。封装完整的BMP-2 /聚乙二醇（PEC）复合物的聚乳酸-乙醇酸接枝的透明质酸（HA-PLGA）成功地进行了组织工程支架的固体自由形式制造（SFF） 。 HA-PLGA共轭物是通过己二酸二酰肼修饰的HA（HA-ADH）与被NX-二环己基碳二亚胺（DCC）和N-羟基琥珀酰亚胺（NHS）活化的PLGA的共轭反应在二甲亚砜（DMSO）中合成的。 BMP-2与PEG混合，通过SFF将其封装在HA-PLGA缀合物的PLGA域中，以制备组织工程支架。体外释放测试证实完整的BMP-2从支架中持续释放长达一个月。确认成骨细胞生长增强，并在细胞中表达高水平的碱性磷酸酶（ALP），骨钙素（OC）和osterix（OSX）基因后，植入HA-PLGA / PEG / BMP-2支架Sprague Dawley（SD）大鼠的颅骨缺损。微型计算机断层扫描（jiCT）以及使用Masson三色，苏木精和曙红（H＆E）染色的组织学分析显示，HA-PLGA / PEG / BMP-2共混物的支架上具有有效的骨再生。

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  • 来源
    《Advanced Functional Materials》 |2011年第15期|p.2906-2912|共7页
  • 作者

    Jung Kyu Park; Jin-Hyung Shim; Kyung Shin Kang; Junseok Yeom; Ho Sang Jung; Jong Young Kim; Keum Hong Lee; Tae-Ho Kim; Shin-Yoon Kim; Dong-Woo Cho; Sei Kwang Hahn;

  • 作者单位

    Department of Materials Science and Engineering Pohang University of Science and Technology (POSTECH) San 31, Hyoja-dong, Nam-gu, Pohang, Kyungbuk 790-784, Korea;

    Department of Mechanical Engineering and/or Division of Integrative Biosciences and Biotechnology POSTECH, Korea;

    Department of Mechanical Engineering and/or Division of Integrative Biosciences and Biotechnology POSTECH, Korea;

    Department of Materials Science and Engineering Pohang University of Science and Technology (POSTECH) San 31, Hyoja-dong, Nam-gu, Pohang, Kyungbuk 790-784, Korea;

    Department of Materials Science and Engineering Pohang University of Science and Technology (POSTECH) San 31, Hyoja-dong, Nam-gu, Pohang, Kyungbuk 790-784, Korea;

    Department of Mechanical Engineering Andong National University 388 Songchun-dong, Andong, Kyungbuk 760-749, Korea;

    Kyungpook National University Hospital Kyungpook National University 101 Dongin-dong 2 Ga, Jung-gu, Daegu, 700-422, Korea;

    Department of Medicine School of Medicine Kyungpook National University 101 Dongin-dong 2 Ga, Jung-gu, Daegu, 700-422, Korea;

    Kyungpook National University Hospital Kyungpook National University 101 Dongin-dong 2 Ga, Jung-gu, Daegu, 700-422, Korea;

    Department of Mechanical Engineering and/or Division of Integrative Biosciences and Biotechnology POSTECH, Korea;

    Department of Materials Science and Engineering Pohang University of Science and Technology (POSTECH) San 31, Hyoja-dong, Nam-gu, Pohang, Kyungbuk 790-784, Korea;

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