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Stereolithography-Based Hydrogel Microenvironmerits to Examine Cellular Interactions

机译：基于立体光刻技术的水凝胶微环境研究细胞相互作用

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摘要

A spatially organized three-dimensional (3D) co-culture of multiple cell types is required to recapitulate cellular interactions and microenvironments in complex tissues. Although there are limited reports for 3D patterning of cells and materials, approaches to examine functional interactions of 3D spatially patterned multiple cell types are lacking entirely. This is mostly due to difficulties in controlling the physical arrangement of cells in a 3D matrix and the physical properties of the cell-encapsulating matrix, while keeping the cells alive and functional for extended periods of time. In this study, an automated maskless fabrication technique is combined with a tunable polymer blend to spatially organize primary hippocampus neurons (HNs) and skeletal muscle myoblast cells (MCs) in a 3D hydrogel matrix with tunable mechanical and degradation properties. The spatial organization of these multiple cell types revealed that the presence of MCs resulted in increased cholinergic functionality of the HNs, as quantified by their choline acetyltransferase activity. The presence of a factor alone is not sufficient, but its spatiotemporal control is necessary; a condition that is possibly true for many cellular interactions. Therefore, the system described here offers a different approach to examine such previously unknown interactions. The approach proposed in this study can be used to examine interactions between many different cell types and shift the 3D fabrication paradigm to a next level, which is to fabricate tissues that are not only viable but also functional.
机译：需要多种细胞类型的空间组织的三维（3D）共培养，以概括复杂组织中的细胞相互作用和微环境。尽管关于细胞和材料的3D图案化的报道有限，但完全缺乏检查3D空间图案化的多种细胞类型的功能相互作用的方法。这主要是由于难以控制3D矩阵中的单元格的物理排列和单元格封装矩阵的物理属性，同时又无法长时间保持单元格的存活和功能。在这项研究中，自动无掩模制造技术与可调聚合物共混物相结合，在具有可调机械和降解特性的3D水凝胶基质中空间组织原代海马神经元（HN）和骨骼肌成肌细胞（MC）。这些多种细胞类型的空间组织显示，MC的存在导致HN的胆碱能功能增强（如其胆碱乙酰转移酶活性所定量）。仅存在一个因素是不够的，但必须进行时空控制。对于许多细胞相互作用而言可能是正确的条件。因此，此处描述的系统提供了一种不同的方法来检查这种先前未知的交互。这项研究中提出的方法可用于检查许多不同细胞类型之间的相互作用，并将3D制造范例转移到一个新的水平，从而制造不仅可行而且功能强大的组织。

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  • 来源
    《Advanced Functional Materials》 |2011年第19期|p.3642-3651|共10页
  • 作者

    Pinar Zorlutuna; Jae Hyun Jeong; Hyunjoon Kong; Rashid Bashir;

  • 作者单位

    Department of Electrical and Computer Engineering University of Illinois at Urbana-Champaign Urbana, Illinois 61801, USA,Micro and Nanotechnology Laboratory University of Illinois at Urbana-Champaign Urbana, Illinois 61801, USA;

    rnDepartment of Chemical and Biomolecular Engineering University of Illinois at Urbana-Champaign Urbana, Illinois 61801, USA;

    rnDepartment of Chemical and Biomolecular Engineering University of Illinois at Urbana-Champaign Urbana, Illinois 61801, USA,Micro and Nanotechnology Laboratory University of Illinois at Urbana-Champaign Urbana, Illinois 61801, USA,Institute of Cenomic Biology University of Illinois at Urbana-Champaign Urbana, Illinois 61801, USA;

    rnDepartment of Electrical and Computer Engineering University of Illinois at Urbana-Champaign Urbana, Illinois 61801, USA,Department of Bioengineering University of Illinois at Urbana-Champaign Urbana, Illinois 61801, USA,Micro and Nanotechnology Laboratory University of Illinois at Urbana-Champaign Urbana, Illinois 61801, USA,Institute of Cenomic Biology University of Illinois at Urbana-Champaign Urbana, Illinois 61801, USA;

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