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首页> 外文期刊>Advanced Functional Materials >Encapsulation of RNA Molecules in BSA Microspheres and Internalization into Trypanosoma Brucei Parasites and Human U2OS Cancer Cells
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Encapsulation of RNA Molecules in BSA Microspheres and Internalization into Trypanosoma Brucei Parasites and Human U2OS Cancer Cells

机译：BSA微球中RNA分子的包封和内在化锥虫布鲁氏菌寄生虫和人类U2OS癌细胞

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摘要

RNA was encapsulated in bovine serum albumin (BSA) microspheres using a one-step sonochemical process from an water-oil solvent biphasic system. Confocal microcoscopy and fluorescence-activated cell sorting indicate that a CY3-RNA (RNA labeled with red fluorescent indocarbocyanine Cy3 dye) sphere is encapsulated in the BSA outer sphere. The diameter of the sphere depends on the number of nucleotides of the RNA, ranging from 0.63 to 2.74 μm. Total RNA (t-RNA) was used as a prototype for the future small interfering RNA (siRNA) delivery. A very broad size distribution characterizes the RNA spheres and therefore, among the loaded BSA spheres, there were sufficiently small spheres to be successfully introduced into trypanosoma brucei parasites and human osteosarcoma U2OS cancer cells.
机译：使用一步法从水油溶剂双相系统中进行超声化学处理，将RNA包裹在牛血清白蛋白（BSA）微球中。共聚焦显微镜和荧光激活的细胞分选表明，CY3-RNA（用红色荧光吲哚羰花青Cy3染料标记的RNA）球被封装在BSA外球中。球体的直径取决于RNA的核苷酸数，范围为0.63至2.74μm。总RNA（t-RNA）被用作未来小干扰RNA（siRNA）交付的原型。 RNA球具有非常宽的尺寸分布，因此，在负载的BSA球中，有足够小的球可以成功引入布鲁氏锥虫和人骨肉瘤U2OS癌细胞。

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  • 来源
    《Advanced Functional Materials》 |2011年第19期|p.3659-3666|共8页
  • 作者

    Ulyana Shimanovich; Itai Dov Tkacz; Dror Eliaz; Artur Cavaco-Paulo; Shulamit Michaeli; Aharon Gedanken;

  • 作者单位

    Department of Chemistry and Kanbar Laboratory for Nanomaterials Bar-llan University Center for Advanced Materials and Nanotechnology Bar-llan University Ramat-Can 52900, Israel;

    rnMina and Everard Goodman Faculty of Life Sciences Bar-llan University Ramat-Gan, 52900, Israel;

    rnMina and Everard Goodman Faculty of Life Sciences Bar-llan University Ramat-Gan, 52900, Israel;

    rnUniversity of Minho Textile Engineering Department P-4800058 Guimaraes, Portugal;

    rnMina and Everard Goodman Faculty of Life Sciences Bar-llan University Ramat-Gan, 52900, Israel;

    rnDepartment of Chemistry and Kanbar Laboratory for Nanomaterials Bar-llan University Center for Advanced Materials and Nanotechnology Bar-llan University Ramat-Can 52900, Israel;

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