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首页> 外文期刊>Advanced Functional Materials >Novel Magnetic Hydroxyapatite Nanoparticles as Non-Viral Vectors for the Clial Cell Line-Derived Neurotrophic Factor Gene
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Novel Magnetic Hydroxyapatite Nanoparticles as Non-Viral Vectors for the Clial Cell Line-Derived Neurotrophic Factor Gene

机译：胶体细胞源性神经营养因子基因的新型磁性羟基磷灰石纳米粒子作为非病毒载体。

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摘要

Nanoparticles (NPs) of synthetic hydroxyapatite (Hap) and natural bone mineral (NBM) are rendered magnetic by treatment with iron ions using a wet-chemical process. The magnetic NPs (mNPs), which are about 300 nm in diameter, display superparamagnetic properties in a superconducting quantum interference device, with a saturation magnetization of about 30 emu g~(-1). X-ray diffraction and transmission electron microscopy reveal that the magnetic properties of the NPs are the result of the hetero-epitaxial growth of magnetite on the Hap and NBM crystallites. The mNPs display a high binding affinity for plasmid DNA in contrast to magnetite NPs which do not bind the plasmid well. The mHap and mNBM NPs result in substantial increases in the transfection of rat marrow-derived mesenchymal stem cells with the gene for glial cell line-derived neurotrophic factor (CDNF), with magnetofection compared to transfection in the absence of a magnet The amount of CDNF recovered in the medium approaches therapeutic levels despite the small amount of plasmid delivered by the NPs.
机译：合成羟基磷灰石（Hap）和天然骨矿物质（NBM）的纳米颗粒（NPs）通过使用湿化学工艺用铁离子处理而具有磁性。直径约为300 nm的磁性NP（mNP）在超导量子干涉装置中显示出超顺磁性，饱和磁化强度约为30 emu g〜（-1）。 X射线衍射和透射电子显微镜显示，NPs的磁性是Hap和NBM晶粒上磁铁矿异质外延生长的结果。与不能很好地结合质粒的磁铁矿NPs相比，mNPs对质粒DNA具有很高的结合亲和力。 mHap和mNBM NP导致大鼠骨髓间充质干细胞转染神经胶质细胞系神经营养因子（CDNF）的基因的转染显着增加，与无磁铁转染相比，磁转染的转染率更高。尽管NPs递送的质粒很少，但是在培养基中回收的RNA仍接近治疗水平。

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  • 来源
    《Advanced Functional Materials》 |2010年第1期|67-77|共11页
  • 作者

    Hsi-Chin Wu; Tzu-Wei Wang; Martha C. Bohn; Feng-Huei Lin; Myron Spector;

  • 作者单位

    Tissue Engineering Laboratories VA Boston Healthcare System 150 S. Huntington Ave. Boston, MA, 02130 (USA) Institute of Biomedical Engineering National Taiwan University No. 1, Sec. 1, Jen-Ai Rd., Jongjheng District Taipei City 100 (Taiwan);

    rnTissue Engineering Laboratories VA Boston Healthcare System 150 S. Huntington Ave. Boston, MA, 02130 (USA) Department of Orthopaedic Surgery Brigham and Women's Hospital Harvard Medical School 75 Francis St. Boston, MA, 02115 (USA) Department of Materials Sciences and Engineering National Tsing Hua University No. 101, Section 2, Kuang-Fu Road Hsinchu 30013 (Taiwan);

    Children's Memorial Research Center Northwestern University 2430 N. Halsted Chicago, IL 60614 (USA);

    Institute of Biomedical Engineering National Taiwan University No. 1, Sec. 1, Jen-Ai Rd., Jongjheng District Taipei City 100 (Taiwan);

    Tissue Engineering Laboratories VA Boston Healthcare System 150 S. Huntington Ave. Boston, MA, 02130 (USA) Department of Orthopaedic Surgery Brigham and Women's Hospital Harvard Medical School 75 Francis St. Boston, MA, 02115 (USA);

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