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Rapid Generation of Biologically Relevant Hydrogels Containing Long-Range Chemical Gradients

机译：快速生成含有远距离化学梯度的生物相关水凝胶

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摘要

Many biological processes are regulated by gradients of bioactive chemicals. Thus, the generation of materials with embedded chemical gradients may be beneficial for understanding biological phenomena and generating tissue-mimetic constructs. Here a simple and versatile method to rapidly generate materials containing centimeter-long gradients of chemical properties in a microfluidic channel is described. The formation of a chemical gradient is initiated by a passive-pump-induced forward flow and further developed during an evaporation-induced backward flow. The gradient is spatially controlled by the backward flow time and the hydrogel material containing the gradient is synthesized via photopolymerization. Gradients of a cell-adhesion ligand, Arg-Gly-Asp-Ser (RCDS), are incorporated in poly(ethylene glycol)-diacrylate (PEG-DA) hydrogels to test the response of endothelial cells. The cells attach and spread along the hydrogel material in a manner consistent with the RCDS-gradient profile. A hydrogel containing a PEG-DA concentration gradient and constant RCDS concentration is also shown. The morphology of cells cultured on such hydrogel changes from round in the lower PEC-DA concentration regions to well-spread in the higher PEC-DA concentration regions. This approach is expected to be a valuable tool to investigate the cell-material interactions in a simple and high-throughput manner and to design graded biomimetic materials for tissue engineering applications.
机译：许多生物过程受生物活性化学物质的梯度调节。因此，具有嵌入式化学梯度的材料的产生对于理解生物学现象和产生组织模拟构造可能是有益的。在此描述了一种简单而通用的方法，用于在微流体通道中快速生成包含厘米级化学性质梯度的材料。化学梯度的形成是由被动泵引起的前向流动引发的，并在蒸发引起的向后流动期间进一步发展。该梯度在空间上受反向流动时间的控制，并且包含该梯度的水凝胶材料是通过光聚合合成的。将细胞粘附配体Arg-Gly-Asp-Ser（RCDS）的梯度掺入聚（乙二醇）-二丙烯酸酯（PEG-DA）水凝胶中，以测试内皮细胞的反应。细胞以与RCDS梯度分布一致的方式附着并沿着水凝胶材料扩散。还显示了包含PEG-DA浓度梯度和恒定RCDS浓度的水凝胶。在这种水凝胶上培养的细胞的形态从较低PEC-DA浓度区域的圆形变化为在较高PEC-DA浓度区域的良好分布。该方法有望成为一种以简单且高通量的方式研究细胞与材料之间相互作用的有价值的工具，并为组织工程应用设计分级仿生材料。

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  • 来源
    《Advanced Functional Materials》 |2010年第1期|131-137|共7页
  • 作者

    Jiankang He; Yanan Du; Jose L. Villa-Uribe; Changmo Hwang; Dichen Li; Ali Khademhosseini;

  • 作者单位

    Center for Biomedical Engineering Department of Medicine Brigham and Women's Hospital Harvard Medical School Boston, MA, 02115 (USA) Harvard-MIT Division of Health Sciences and Technology Massachusetts Institute of Technology Cambridge, MA, 02139 (USA) State Key Laboratory of Manufacturing Systems Engineering Xi'an Jiaotong University Xi'an, Shaanxi, 710049 (P. R. China);

    rnCenter for Biomedical Engineering Department of Medicine Brigham and Women's Hospital Harvard Medical School Boston, MA, 02115 (USA) Harvard-MIT Division of Health Sciences and Technology Massachusetts Institute of Technology Cambridge, MA, 02139 (USA);

    rnCenter for Biomedical Engineering Department of Medicine Brigham and Women's Hospital Harvard Medical School Boston, MA, 02115 (USA) Harvard-MIT Division of Health Sciences and Technology Massachusetts Institute of Technology Cambridge, MA, 02139 (USA);

    rnCenter for Biomedical Engineering Department of Medicine Brigham and Women's Hospital Harvard Medical School Boston, MA, 02115 (USA) Harvard-MIT Division of Health Sciences and Technology Massachusetts Institute of Technology Cambridge, MA, 02139 (USA);

    rnState Key Laboratory of Manufacturing Systems Engineering Xi'an Jiaotong University Xi'an, Shaanxi, 710049 (P. R. China);

    rnCenter for Biomedical Engineering Department of Medicine Brigham and Women's Hospital Harvard Medical School Boston, MA, 02115 (USA);

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