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首页> 外文期刊>Advanced Functional Materials >Time Controlled Protein Release from Layer-by-Layer Assembled Multilayer Functionalized Agarose Hydrogels
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Time Controlled Protein Release from Layer-by-Layer Assembled Multilayer Functionalized Agarose Hydrogels

机译：时间控制的蛋白质从多层组装的多层功能化琼脂糖水凝胶中释放

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摘要

Axons of the adult central nervous system exhibit an extremely limited ability to regenerate after spinal cord injury. Experimentally generated patterns of axon growth are typically disorganized and randomly oriented. Support of linear axonal growth into spinal cord lesion sites has been demonstrated using arrays of uniaxial channels, templated with agarose hydrogel, and containing genetically engineered cells that secrete brain-derived neurotrophic factor (BDNF). However, immobilizing neurotrophic factors secreting cells within a scaffold is relatively cumbersome, and alternative strategies are needed to provide sustained release of BDNF from templated agarose scaffolds. Existing methods of loading the drug or protein into hydrogels cannot provide sustained release from templated agarose hydrogels. Alternatively, here it is shown that pH-responsive H-bonded poly(ethylene glycol)(PEG)/poly(acrylic acid)(PAA)/protein hybrid layer-by-layer (LbL) thin films, when prepared over agarose, provided sustained release of protein under physiological conditions for more than four weeks. Lysozyme, a protein similar in size and isoelectric point to BDNF, is released from the multilayers on the agarose and is biologically active during the earlier time points, with decreasing activity at later time points. This is the first demonstration of month-long sustained protein release from an agarose hydrogel, whereby the drug/protein is loaded separately from the agarose hydrogel fabrication process.
机译：成人中枢神经系统的轴突在脊髓损伤后表现出极其有限的再生能力。实验产生的轴突生长模式通常是杂乱无章的，方向随机的。使用单轴通道阵列，以琼脂糖水凝胶为模板并包含分泌脑源性神经营养因子（BDNF）的基因工程细胞，已证明支持线性轴突生长进入脊髓病变部位。然而，将分泌细胞的神经营养因子固定化在支架中相对较麻烦，并且需要替代策略以提供从模板化琼脂糖支架中持续释放的BDNF。将药物或蛋白质装载到水凝胶中的现有方法不能提供从模板化琼脂糖水凝胶的持续释放。或者，此处显示了在琼脂糖上制备时，可提供pH响应的氢键键合的聚（乙二醇）（PEG）/聚（丙烯酸）（PAA）/蛋白质杂化层（LbL）薄膜。在生理条件下持续释放蛋白质超过四个星期。溶菌酶是一种大小和等电点与BDNF相似的蛋白质，它从琼脂糖上的多层膜中释放出来，在较早的时间点具有生物活性，而在较晚的时间点活性降低。这是从琼脂糖水凝胶中持续一个月的蛋白质持续释放的第一个证明，其中药物/蛋白质与琼脂糖水凝胶的制造过程分开装载。

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  • 来源
    《Advanced Functional Materials》 |2010年第2期|247-258|共12页
  • 作者

    Sumit Mehrotra; Daniel Lynam; Ryan Maloney; Kendell M. Pawelec; Mark H. Tuszynski; Ilsoon Lee; Christina Chan; Jeffrey Sakamoto;

  • 作者单位

    Department of Chemical Engineering and Materials Science Michigan State University East Lansing, Michigan 48824, MI (USA);

    Department of Chemical Engineering and Materials Science Michigan State University East Lansing, Michigan 48824, MI (USA);

    Department of Chemical Engineering and Materials Science Michigan State University East Lansing, Michigan 48824, MI (USA);

    Department of Chemical Engineering and Materials Science Michigan State University East Lansing, Michigan 48824, MI (USA);

    Director-Center for Neural Repair Department of Neurosciences University of California San Diego 92093, CA (USA);

    Department of Chemical Engineering and Materials Science Michigan State University East Lansing, Michigan 48824, MI (USA);

    Department of Chemical Engineering and Materials Science Michigan State University East Lansing, Michigan 48824, MI (USA);

    Department of Chemical Engineering and Materials Science Michigan State University East Lansing, Michigan 48824, MI (USA);

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