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A Graphene Nanoprobe for Rapid, Sensitive, and Multicolor Fluorescent DNA Analysis

机译：用于快速，灵敏和多色荧光DNA分析的石墨烯纳米探针

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摘要

Coupling nanomaterials with biomolecular recognition events represents a new direction in nanotechnology toward the development of novel molecular diagnostic tools. Here a graphene oxide (GO)-based multicolor fluorescent DNA nanoprobe that allows rapid, sensitive, and selective detection of DNA targets in homogeneous solution by exploiting interactions between GO and DNA molecules is reported. Because of the extraordinarily high quenching efficiency of GO, the fluorescent ssDNA probe exhibits minimal background fluorescence, while strong emission is observed when it forms a double helix with the specific targets, leading to a high signal-to-background ratio. Importantly, the large planar surface of GO allows simultaneous quenching of multiple DNA probes labeled with different dyes, leading to a multicolor sensor for the detection of multiple DNA targets in the same solution. It is also demonstrated that this GO-based sensing platform is suitable for the detection of a range of analytes when complemented with the use of functional DNA structures.
机译：纳米材料与生物分子识别事件的耦合代表了纳米技术向新型分子诊断工具发展的新方向。本文报道了一种基于氧化石墨烯（GO）的多色荧光DNA纳米探针，该探针通过利用GO和DNA分子之间的相互作用，可以在均相溶液中快速，灵敏和选择性地检测DNA靶标。由于GO的猝灭效率极高，荧光ssDNA探针显示出最小的背景荧光，而当它与特定靶标形成双螺旋时，观察到强发射，从而导致很高的信噪比。重要的是，GO的大平面允许同时淬灭多个用不同染料标记的DNA探针，从而导致了一种用于检测同一溶液中多个DNA靶标的多色传感器。还证明了，当使用功能性DNA结构进行补充时，这种基于GO的传感平台适用于检测多种分析物。

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  • 来源
    《Advanced Functional Materials》 |2010年第3期|453-459|共7页
  • 作者

    Shijiang He; Bo Song; Di Li; Changfeng Zhu; Wenpeng Qi; Yanqin Wen; Lihua Wang; Shiping Song; Haiping Fang; Chunhai Fan;

  • 作者单位

    Laboratory of Physical Biology Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai 201800 (China);

    Laboratory of Physical Biology Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai 201800 (China);

    Laboratory of Physical Biology Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai 201800 (China);

    Laboratory of Physical Biology Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai 201800 (China);

    Laboratory of Physical Biology Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai 201800 (China);

    Laboratory of Physical Biology Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai 201800 (China);

    Laboratory of Physical Biology Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai 201800 (China);

    Laboratory of Physical Biology Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai 201800 (China);

    Laboratory of Physical Biology Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai 201800 (China);

    Laboratory of Physical Biology Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai 201800 (China);

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