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Enhancement of Aggregation-Induced Emission in Dye-Encapsulating Polymeric Micelles for Bioimaging

机译：增强染料封装聚合物胶束中生物成像的聚集诱导发射。

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摘要

Three amphiphilic block copolymers are employed to form polymeric micelles and function as nanocarriers to disperse hydrophobic aggregation-induced emission (AIE) dyes, 1,1,2,3,4,5-hexaphenylsilole (HPS) and/or bis(4-(N-(1-naphthyl) phenylamino)-phenyl)fumaronitrile (NPAFN), into aqueous solution for biological studies. Compared to their virtually non-emissive properties in organic solutions, the fluorescence intensity of these AIE dyes has increased significantly due to the spatial confinement that restricts intramolecular rotation of these dyes and their better compatibility in the hydrophobic core of polymeric micelles. The effect of the chemical structure of micelle cores on the photophysical properties of AIE dyes are investigated, and the fluorescence resonance energy transfer (FRET) from the green-emitting donor (HPS) to the red-emitting acceptor (NPAFN) is explored by co-encapsulating this FRET pair in the same micelle core. The highest fluorescence quantum yield (～62%) could be achieved by encapsulating HPS aggregates in the micelles. Efficient energy transfer (>99%) and high amplification of emission (as high as 8 times) from the NPAFN acceptor could also be achieved by spatially confining the HPS/NPAFN FRET pair in the hydrophobic core of polymeric micelles. These micelles could be successfully internalized into the RAW 264.7 cells to demonstrate high-quality fluorescent images and cell viability due to improved quantum yield and reduced cytotoxicity.
机译：三种两亲嵌段共聚物用于形成聚合物胶束并用作纳米载体以分散疏水性聚集诱导发射（AIE）染料，1,1,2,3,4,5-六苯基甲硅烷基（HPS）和/或双（4-（将N-（1-萘基苯基氨基）-苯基）富马腈（NPAFN）放入水溶液中进行生物学研究。与它们在有机溶液中的几乎非发射性相比，由于空间限制了这些染料的分子内旋转以及它们在聚合物胶束的疏水核中的更好的相容性，这些AIE染料的荧光强度已显着提高。研究了胶束核心的化学结构对AIE染料光物理性质的影响，并探索了从绿色发光供体（HPS）到红色发光受体（NPAFN）的荧光共振能量转移（FRET）。 -将此FRET对封装在相同的胶束核心中。通过将HPS聚集体包裹在胶束中，可以获得最高的荧光量子产率（〜62％）。通过将HPS / NPAFN FRET对在空间上限制在聚合物胶束的疏水核中，也可以实现NPAFN受体的有效能量转移（> 99％）和高发射放大率（高达8倍）。这些胶束可以成功地内化到RAW 264.7细胞中，以显示高质量的荧光图像和细胞活力，这归因于量子产率的提高和细胞毒性的降低。

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  • 来源
    《Advanced Functional Materials》 |2010年第9期|P.1413-1423|共11页
  • 作者

    Wen-Chung Wu; Ching-Yi Chen; Yanqing Tian; Sei-Hum Jang; Yuning Hong; Yang Liu; Rongrong Hu; Ben Zhong Tang; Yi-Ting Lee; Chin-Ti Chen; Wen-Chang Chen; Alex K.-Y. Jen;

  • 作者单位

    Department of Materials Science and Engineering, Box 352120 University of Washington Seattle, WA 98195-2120 (USA);

    rnDepartment of Materials Science and Engineering, Box 352120 University of Washington Seattle, WA 98195-2120 (USA);

    rnDepartment of Materials Science and Engineering, Box 352120 University of Washington Seattle, WA 98195-2120 (USA);

    rnDepartment of Materials Science and Engineering, Box 352120 University of Washington Seattle, WA 98195-2120 (USA);

    rnDepartment of Chemistry The Hong Kong University of Science & Technology Clear Water Bay, Kowloon, Hong Kong (China);

    rnDepartment of Chemistry The Hong Kong University of Science & Technology Clear Water Bay, Kowloon, Hong Kong (China);

    rnDepartment of Chemistry The Hong Kong University of Science & Technology Clear Water Bay, Kowloon, Hong Kong (China);

    rnDepartment of Chemistry The Hong Kong University of Science & Technology Clear Water Bay, Kowloon, Hong Kong (China);

    rnInstitute of Chemistry Academia Sinica Taipei 115 (Taiwan);

    rnInstitute of Chemistry Academia Sinica Taipei 115 (Taiwan);

    rnDepartment of Chemical Engineering and Institute of Polymer Science and Engineering National Taiwan University Taipei 106 (Taiwan);

    rnDepartment of Materials Science and Engineering, Box 352120 University of Washington Seattle, WA 98195-2120 (USA);

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