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Nanogold-Loaded Sharp-Edged Carbon Bullets as Plant-Gene Carriers

机译：纳米金负载的锋利碳弹作为植物基因载体。

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摘要

The higher DNA delivery efficiency into plants by gold nanoparticles embedded in sharp carbonaceous carriers is demonstrated. These nanogold-embedded carbon matrices are prepared by heat treatment of biogenic intracellular gold nanoparticles. The DNA-delivery efficiency is tested on a model plant, Nicotiana tabacum, and is further extended to the monocot, Oryza sativa, and a hard dicot tree species, Leucaena leucocephala. These materials reveal good dispersion of the transport material, producing a greater number of GUS foci per unit area. The added advantages of the composite carrier are the lower plasmid and gold requirements. Plant-cell damage with the carbon-supported particles is very minimal and can be gauged from the increased plant regeneration and transformation efficiency compared with that of the commercial micrometer-sized gold particles. This is ascribed to the sharp edges that the carbon supports possess, which lead to better piercing capabilities with minimum damage.
机译：嵌入在尖锐的碳质载体中的金纳米颗粒具有更高的向植物的DNA传递效率。这些纳米金嵌入的碳基质是通过对生物细胞内金纳米颗粒进行热处理而制备的。 DNA传递效率在模型植物烟草（Nicotiana tabacum）上进行了测试，并进一步扩展到单子叶植物Oryza sativa和硬双子叶植物树种Leucaena leucocephala。这些材料显示出运输材料的良好分散性，每单位面积产生更多数量的GUS病灶。复合载体的附加优点是较低的质粒和金需求。碳负载颗粒对植物细胞的损害非常小，与商用微米级金颗粒相比，可以通过增加的植物再生和转化效率来衡量。这归因于碳载体所具有的锋利边缘，这导致更好的穿刺能力且损害最小。

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