• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Advanced Functional Materials >Conjugated Polyelectrolytes As Light-up Macromolecular Probes For Heparin Sensing
【24h】

Conjugated Polyelectrolytes As Light-up Macromolecular Probes For Heparin Sensing

机译：共轭聚电解质作为肝素传感的发光高分子探针

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Two cationic poly(fluorene-alt-benzothiadiazole)s with different side chains are designed and synthesized. Both polymers show low fluorescence in aqueous solution due to the charge-transfer character of the polymer's excited states. Fluorescence turn-on biosensors for heparin detection and quantification are developed, taking advantage of complexation-induced aggregation, which increases the polymer fluorescence in aqueous solution. It is found that good polymer water-solubility is beneficial to the sensitivity and fluorescence contrast of the heparin turn-on sensor as a result of the low fluorescence background. Moreover, stronger complexation between the polymer/heparin leads to a substantially larger fluorescence increase in the presence of heparin relative to that in the presence of its analog, hyaluronic acid (HA), allowing discrimination of heparin from HA. Heparin quantification with a practical calibration range covering the whole therapeutic dosing levels (0.2-8 U mL~(-1)) is realized based on the polymer with good water-solubility. This investigation provides a new insight for designing conjugated polymers with a light-up signature for biomolecular sensing.
机译：设计并合成了两种侧链不同的阳离子聚（芴-alt-苯并噻二唑）。由于聚合物激发态的电荷转移特性，两种聚合物在水溶液中均显示低荧光。利用络合诱导的聚集作用，开发了用于肝素检测和定量的荧光开启生物传感器，从而增加了水溶液中的聚合物荧光。发现由于低的荧光背景，良好的聚合物水溶性对肝素开启传感器的灵敏度和荧光对比度有利。此外，相对于存在其类似物透明质酸（HA）的情况，在肝素存在下，聚合物/肝素之间更强的络合作用会导致荧光增加明显更多，从而可以区分肝素与HA。基于具有良好水溶性的聚合物，可以实现覆盖整个治疗剂量水平（0.2-8 U mL〜（-1））的实用校准范围内的肝素定量。这项研究为设计具有发光标记的生物分子传感共轭聚合物提供了新的见识。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号