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首页> 外文期刊>Advanced Functional Materials >Enhanced Osteoblast Adhesion to Epoxide-Functionalized Surfaces
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Enhanced Osteoblast Adhesion to Epoxide-Functionalized Surfaces

机译：增强的成骨细胞对环氧功能化表面的粘附力

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摘要

As an alternative to expensive extracellular matrix (ECM) proteins generally applied as coatings in Petri dishes used for cell binding, an innovative system based on epoxide-functionalized monolayers capable of protein binding is proposed. Since cells bind to material surfaces through proteins, protein-binding surfaces should also promote cell binding. Here we investigate how the cell-binding properties of an epoxide-functionalized surface compares with ECM protein gel coated surfaces and tissue culture polystyrene control surfaces. Glass surfaces are functionalized with glycidoxypropyltriethoxysilane (GOPS), which results in an epoxide-functionalized surface capable of binding proteins through an epoxide-amine reaction. Advancing contact angle measurements and atomic force microscopy measurements confirm the formation of a homogeneous GOPS monolayer. This monolayer is micropatterned with fluorescein-labeled ECM protein gel by microcontact printing (μCP). Confocal laser scanning microscopy (CLSM) shows accurately transferred ECM protein gel micropatterns. Osteoblasts that are seeded on these micropatterned substrates show a clear preference for adhering to the epoxide-functionalized areas. The morphology of these cultured osteoblasts is needle-like with high aspect ratios. As controls, osteoblasts are cultured on GOPS-functionalized surfaces, unstructured ECM protein gel surfaces, and tissue culture polystyrene (TCPS). The GOPS surfaces demonstrate a drastic increase in cell adhesion after 2 h, whilst the other tests show no adverse effects of this surface on the osteoblasts as compared to ECM and TCPS. CLSM shows healthy cell morphologies on each surface. It is demonstrated for the first time that epoxide groups outperform ECM protein gel in cell adhesion, thereby providing new routes for cost-effective coatings that improve biocompat-ibility as well as exciting, new methodologies to control and direct cell adhesion.
机译：作为昂贵的细胞外基质（ECM）蛋白的替代品，该蛋白通常用作细胞结合培养皿中的涂层，提出了一种基于能够与蛋白质结合的环氧官能化单分子膜的创新系统。由于细胞通过蛋白质与物质表面结合，因此蛋白质结合表面也应促进细胞结合。在这里，我们研究了环氧官能化表面与ECM蛋白凝胶涂层表面和组织培养聚苯乙烯对照表面的细胞结合特性。玻璃表面用环氧丙氧基丙基三乙氧基硅烷（GOPS）进行了功能化，从而形成了一种能够通过环氧-胺反应结合蛋白质的环氧官能化表面。先进的接触角测量和原子力显微镜测量证实了均匀的GOPS单层的形成。通过微接触印刷（μCP）用荧光素标记的ECM蛋白凝胶对该图案进行微图案化。共聚焦激光扫描显微镜（CLSM）显示准确转移的ECM蛋白凝胶显微图案。播种在这些微图案化基质上的成骨细胞表现出明显的粘附于环氧官能化区域的偏好。这些培养的​​成骨细胞的形态为高纵横比的针状。作为对照，将成骨细胞培养在GOPS功能化的表面，非结构化ECM蛋白凝胶表面和组织培养聚苯乙烯（TCPS）上。 GOPS表面显示2小时后细胞粘附力急剧增加，而其他测试表明，与ECM和TCPS相比，该表面对成骨细胞没有不利影响。 CLSM在每个表面上都显示出健康的细胞形态。首次证明环氧基团在细胞粘附方面胜过ECM蛋白凝胶，从而为经济有效的涂层提供了新途径，该涂层可改善生物相容性以及令人兴奋的新方法来控制和指导细胞粘附。

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