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首页> 外文期刊>Advanced Functional Materials >Gold Nanoparticle-Colloidal Carbon Nanosphere Hybrid Material: Preparation, Characterization, and Application for an Amplified Electrochemical Immunoassay
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Gold Nanoparticle-Colloidal Carbon Nanosphere Hybrid Material: Preparation, Characterization, and Application for an Amplified Electrochemical Immunoassay

机译：金纳米颗粒-胶体碳纳米球杂化材料：制备，表征和应用的放大的电化学免疫测定。

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摘要

A rapid microwave-hydrothermal method has been developed to prepare monodisperse colloidal carbon nanospheres from glucose solution, and gold nanoparticles (AuNPs) are successfully assembled on the surface of the colloidal carbon nanospheres by a self-assembly approach. The resulting AuNP/colloidal carbon nanosphere hybrid material (AuNP/C) has been characterized and is expected to offer a promising template for biomolecule immobilization and biosensor fabrication because of its satisfactory chemical stability and the good biocompatibility of AuNPs. Herein, as an example, it is demonstrated that the as-prepared AuNP/C hybrid material can be conjugated with horseradish peroxidase-labeled antibody (HRP-Ab_2) to fabricate HRP-Ab_2-AuNP/C bioconjugates, which can then be used as a label for the sensitive detection of protein. The amperometric immunosensor fabricated on a carbon nanotube-modified glass carbon electrode was very effective for antibody immobilization. The approach provided a linear response range between 0.01 and 250ng mL~(-1) with a detection limit of 5.6 pg mL~(-1). The developed assay method was versatile, offered enhanced performances, and could be easily extended to other protein detection as well as DNA analysis.
机译：已经开发了一种快速微波-水热法从葡萄糖溶液制备单分散胶体碳纳米球，金纳米颗粒（AuNPs）通过自组装方法成功地组装在胶体碳纳米球的表面。所得的AuNP /胶态碳纳米球杂化材料（AuNP / C）已被表征，由于其令人满意的化学稳定性和AuNPs的良好生物相容性，有望为生物分子固定和生物传感器制造提供有希望的模板。在此，作为例子，证明了可以将制备的AuNP / C杂化材料与辣根过氧化物酶标记的抗体（HRP-Ab_2）缀合以制备HRP-Ab_2-AuNP / C生物缀合物，然后可以将其用作用于敏感检测蛋白质的标签。在碳纳米管修饰的玻璃碳电极上制造的安培免疫传感器对于固定抗体非常有效。该方法提供的线性响应范围为0.01至250ng mL〜（-1），检测极限为5.6 pg mL〜（-1）。发达的检测方法用途广泛，性能增强，可以轻松扩展到其他蛋白质检测和DNA分析中。

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