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首页> 外文期刊>Advanced Functional Materials >SERS Aptatags: New Responsive Metallic Nanostructures for Heterogeneous Protein Detection by Surface Enhanced Raman Spectroscopy
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SERS Aptatags: New Responsive Metallic Nanostructures for Heterogeneous Protein Detection by Surface Enhanced Raman Spectroscopy

机译：SERS Aptatags：用于通过表面增强拉曼光谱检测异质蛋白的新型响应金属纳米结构

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We report here "aptatags," which consist of aptamer-modified silver nanoparticles (NPs) held together by an optical reporter. It is possible to use these materials to design a heterogeneous method for protein identification that takes advantage of the Raman signal enhancement by metallic nanostructures and the recognition capabilities of aptamers. Aptatags are formed by linking silver NPs with an organic dithiol molecule, followed by surface modification with thiolated single-stranded DNA (ssDNA) corresponding to the sequence of the aptamer probe. The sensing surface involves a silver layer containing the thiolated capturing aptamer and mercaptohexadecanoic acid to minimize nonspecific binding. The overall process provides excellent selectivity and sensitivity. Detailed characterization of the sensing surface by SERS maps and atomic force microscopy was carried out to understand how structural features lead to signal generation.
机译：我们在这里报告“ aptatags”，它由光学报告基因结合在一起的适体修饰的银纳米颗粒（NPs）组成。可以使用这些材料来设计用于蛋白质鉴定的异构方法，该方法利用了金属纳米结构对拉曼信号的增强作用以及适体的识别能力。通过将银NP与有机二硫醇分子连接，然后用对应于适体探针序列的硫醇化单链DNA（ssDNA）进行表面修饰，来形成适体标签。感测表面涉及包含硫醇化捕获适体和巯基十六烷酸的银层，以最大程度地减少非特异性结合。整个过程提供了出色的选择性和灵敏度。通过SERS图和原子力显微镜对传感表面进行了详细的表征，以了解结构特征如何导致信号生成。

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