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首页> 外文期刊>Advanced Functional Materials >Artificial antibodies for bioanalyte detection-sensing viruses and proteins
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Artificial antibodies for bioanalyte detection-sensing viruses and proteins

机译：用于生物分析物检测的病毒和蛋白质的人工抗体

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摘要

Molecular imprinting has become an increasingly popular method for the design of artificial antibodies against a variety of analytesi and especially as a method.,for detecting viruses and proteins. Self-assembly of a virus on a stamp enables us to generate patterns on a polymer. Combined with mass-sensitive transducers, the reinclusion of viruses, such as the tobacco mosaic virus (TMV), the human rhinovirus (HRV), and the parapox ovis virus (PPOV), into the patterned polymer layer can be followed directly. Recognition is favored by a geometrical fit between the analyte and the patterned layer, as well as by noncovalent interactions; the former can be observed by the highly selective enrichment of rod-shaped TMV and globular HRV on their respective imprints, showing only negligible cross-sensitivity. The latter is demonstrated by the selective incorporation of different HRV serotypes (HRV-1a and HRV-16) that have the same geometric. dimensions but nonetheless are clearly distinguished from each other. Similar behavior is observed with protein molecules that are about one: order of, magnitude smaller than viruses, having molecular dimensions of a few nanometers. When using proteins for imprinting, the resulting materials usually preferably incorporate into their own template.
机译：分子印迹已成为设计针对多种分析物的人工抗体的一种日益流行的方法，尤其是作为一种检测病毒和蛋白质的方法。邮票上病毒的自组装使我们能够在聚合物上生成图案。结合质量敏感的传感器，可以直接将病毒（例如烟草花叶病毒（TMV），人鼻病毒（HRV）和副痘病毒（PPOV））重新包含到图案化的聚合物层中。分析物与图案化层之间的几何拟合以及非共价相互作用有助于识别。前者可通过棒状TMV和球状HRV在其各自的印记上的高度选择性富集来观察，仅显示可忽略的交叉敏感性。后者通过选择性地并入具有相同几何形状的不同HRV血清型（HRV-1a和HRV-16）来证明。尺寸，但仍然可以清楚地区分。对于蛋白质分子，观察到类似的行为，该蛋白质分子的大小约为几纳米：比病毒小一个数量级，大小小于病毒。当使用蛋白质进行印记时，通常优选将所得材料并入它们自己的模板中。

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