• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Advanced Functional Materials >Live lymphocyte arrays for biosensing
【24h】

Live lymphocyte arrays for biosensing

机译：活淋巴细胞阵列用于生物传感

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Systems designed to sensitively and accurately detect whole pathogen particles, their components, or other proteins diagnostic of infection or disease are of interest as sensors for biodefense and clinical diagnostics. To this end, we examined the potential of a sensing strategy based on live T-cell/B-cell interactions in a biosensor chip format. An approach to fabricate patterned hydrogel microwells functionalized at their bases with antibodies to promote specific immobilization of lymphocytes was developed and used to array single T cells in a regular pattern on a substrate. A sensing platform was created by overlaying arrayed T cells with a confluent layer of antigen-capturing B cells. In this system, a peptide analyte added to the chip was captured by B cells and physically presented to arrayed T cells by B-cell-surface major histocompatibility complex molecules, triggering T cells through their T-cell receptors. T-cell recognition of the target peptide was detected by fluorescence measurements of T-cell intracellular calcium levels, a biochemical read-out of T-cell receptor triggering. We demonstrate that this approach allows rapid, sensitive detection of a model peptide analyte, and that T-cell arrays allow for maximal T-cell/B-cell contacts while simultaneously optimizing single-cell fluorescence detection for analysis of the sensor response. This approach could be of interest for the design of sensing platforms that can detect both peptide fragments and whole intact pathogens, irrespective of surface mutations that might be induced naturally or during "weaponization".
机译：设计用于灵敏和准确地检测整个病原体颗粒，它们的成分或诊断感染或疾病的其他蛋白质的系统作为生物防御和临床诊断的传感器非常有用。为此，我们研究了基于生物传感器芯片形式的实时T细胞/ B细胞相互作用的传感策略的潜力。开发了一种制造图案化的水凝胶微孔的方法，该方法在其碱基处被抗体功能化以促进淋巴细胞的特异性固定，并用于在基板上以规则模式排列单个T细胞。通过将排列的T细胞与抗原捕获B细胞的汇合层重叠来创建传感平台。在该系统中，添加到芯片上的肽分析物被B细胞捕获，并通过B细胞表面主要组织相容性复合物分子物理呈现给阵列T细胞，从而触发T细胞通过其T细胞受体。通过对T细胞细胞内钙水平的荧光测量（T细胞受体触发的生化读数）来检测目标肽的T细胞识别。我们证明了这种方法可以快速，灵敏地检测模型肽分析物，并且T细胞阵列允许最大的T细胞/ B细胞接触，同时优化用于分析传感器响应的单细胞荧光检测。这种方法对于设计可检测肽片段和完整完整病原体的传感平台可能是有意义的，而与天然或“武器化”过程中可能引起的表面突变无关。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号