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Synthesis, functionalization, and bioconjugation of monodisperse, silica-coated gold nanoparticles: Robust bioprobes

机译:单分散,二氧化硅包被的金纳米颗粒的合成,功能化和生物共轭:稳健的生物探针

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Herein, we report an efficient process for preparing monodisperse Au@SiO2 nanoparticles using homogeneous shaking and without the use of surface-coupling silane agents or large stabilizers. The resulting pure-silica surface of the Au@SiO2 nanoparticles is very important for straightforward surface functionalization with different functional groups via well-established silica surface chemistry. Subsequent covalent bioconjugation of the aldehyde-functionalized Au@SiO2 nanoparticles with various biomolecules is successfully employed to make robust nanoprobes for fast, colorimetric DNA and protein detection based on the sequence-specific hybridization properties of DNA and the specific binding affinity between proteins, respectively.
机译:在这里,我们报告了一种有效的方法,该方法使用均匀振荡而不使用表面偶联硅烷试剂或大型稳定剂来制备单分散Au @ SiO2纳米粒子。 Au @ SiO2纳米粒子的纯二氧化硅表面对于通过公认的二氧化硅表面化学方法通过不同的官能团进行简单的表面官能化非常重要。醛官能化的Au @ SiO2纳米粒子与各种生物分子的随后共价生物缀合分别基于DNA的序列特异性杂交特性和蛋白质之间的特异性结合亲和力,成功地用于快速,比色DNA和蛋白质检测的坚固纳米探针。

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